For IC-PCR, rabbit polyclonal antisera
raised against TYLCV-CP and ACMV-CP (provided by Dr.
were coated on the plate by 5 (Ag per well, and AIAT were titrated with 1:100 dilution (Table 2).
Peak F (fractions 46-50) contained at least 2 components in which the later eluting portion (fraction 49) reacted with all 3 BNP antisera
, indicating it is a BNP form with intact C- and N-terminal ends.
Hyperimmune rabbit antisera
against non-typeable strains were produced by the Bacterial Antigens Laboratory in Universidade Estadual de Campinas.
Microscope slides with fixed sections were treated for 2 hours at room temperature with a non immune serum (goat; Sigma), diluted 1:100 in Phosphate Buffered Saline (PBS), and then incubated overnight at 4[degrees]C with four of two primary region-specific antisera
and following the recommended procedure (supplied by Denka Seiken Co.
We isolated immunoglobulin G (IgG) from rabbit antisera
using a two-step procedure as described by McKinney and Parkinson (8).
8] As previously noted, free and complexed A1AT may react differently with different antisera
or assay methods.
All extracts were then tested against the antisera
shown in Table 2.
First, an artifact produced a cross-reaction to mouse antisera
Some people buy commercial antisera
, while others make their own.