injection of 4 mL of normal saline, and at days 28 and 50 with 4 mL and 2 mL of SFBFF antiserum
, respectively, and served as the SFBFF antiserum
treated group (AI).
The membranes were then incubated with the tungro antiserum
at 1 : 100 dilution with an overnight incubation with rocking.
The recombinant protein of SL was used to raise antiserum
in rabbit as described previously .
Cross-reactivities relative to BNP(1-32) 100% were, for the N-terminal antiserum
, BNP (2-32) 0.42%, BNP(3-32) <0.003%; for the commercial antiserum
, BNP(1-32) 100%, BNP(2-32) 27.3%, BNP(3-32) 2.6%; for the C-terminal antiserum
, BNP(19-29) <0.004%, BNP(19-30) <0.004%, BNP(19-31) <0.004%, BNP(19-32) 46.3%, and BNP(19-32)[Ala.sup.33] [BNP(19-32) with an additional nonsequence alanine added at its C terminus] 2%.
As shown in Figure 1(c),nonconjugated albumin, which served as a control, was not detected by any of the anti-altered VWF peptide specific antiserum
(Figure 1(c), represented by lane 8/8').
In the present study, the immunocytochemical localization of regulatory peptides and biogenic amine (5-HT) in the endocrine cells was investigated by use of the monoclonal antiserum
. Consequently, the region specificity of this antibody was tested in the immunocytochemical system and the specificity of this antiserum
was examined by controls.
is the only available medical antidote against snakebite (Calmette, 1894), it does not provide enough protection against venom-induced hemorrhage, necrosis, or nephrotoxicity, and it often produces adverse hypersensitivity reactions (Corrigan et al., 1978; Stahel et al., 1985; Sutherland, 1992).
coli bacteria and the anti-B antiserum
. This year, the experiment was limited to E.
For this, we produced rabbit antiserum
to HDI-adducted keyhole limpet hemocyanin (HDI-KLH).
Immunostaining utilized a 1:1000 dilution of the primary antiserum
, a 1:1000 dilution of HRP-anti rabbit IgG, and 4-chloronaphthol using standard methods for staining Western and dot blots .
The positive reaction to cat antiserum
on the one unifacially retouched flake from DEL-168 indicates protein from a member of the Felidae.
As with all immunological techniques, CIEP requires (1) the production and isolation of antibodies (antiserum
) to known plants and animals, (2) the removal and isolation of plant or animal residue from artifacts or sediment, (3) the exposure of the plant or animal residue to a series of antiserum
, and (4) methods for observing the strength of the reaction of the antiserum
to the unknown residue.