apoenzyme

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apoenzyme

[¦a·pō¦en‚zīm]

(biochemistry)

The protein moiety of an enzyme; determines the specificity of the enzyme reaction.

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An apoenzyme blank was also included in each assay to correct for endogenous PLP that might be present in the apoenzyme extract.

Homogeneous, nonradioactive, enzymatic assay for plasma pyridoxal 5-phosphate

These include loss of reactivatable apoenzyme during chronic deficiency in vivo (7, 8), altered binding of apoenzyme and coenzyme because of the presence of isoenzymes of transketolase (9), and reduced synthesis of apoenzyme in patients with diabetes (10) and liver disease (11).

Vitamin [B.sub.1] status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase activation assay

The cblE class may involve a reducing component of the MS system (84), whereas cblG is thought to be caused by defective MS apoenzyme (85,86).

Homocysteine and vitamins in cardiovascular disease

Biotin, pyridoxal-phosphate, and riboflavin can be covalently bound to the apoenzymes and are called prosthetic groups.