apoenzyme

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apoenzyme

[¦a·pō¦en‚zīm]

(biochemistry)

The protein moiety of an enzyme; determines the specificity of the enzyme reaction.

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An apoenzyme blank was also included in each assay to correct for endogenous PLP that might be present in the apoenzyme extract.

Homogeneous, nonradioactive, enzymatic assay for plasma pyridoxal 5-phosphate

15) and Baines and Davies (16) have suggested that erythrocyte TDP concentrations determined by the apoenzyme recombination technique or by HPLC are a more sensitive index of thiamin status than the measurement of erythrocyte transketolase activity.

Vitamin [B.sub.1] status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase activation assay

The cblE class may involve a reducing component of the MS system (84), whereas cblG is thought to be caused by defective MS apoenzyme (85,86).