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Early fertilization events in the sexual and aposporous egg apparatus of Pennisetum ciliare (L.) Link.
This may explain the occurrence of some of the sexual and aposporous meiocytes in which callose was restricted to the wall closest to the micropyle.
Meiosis in both sexual and aposporous buffelgrass (Pennisetum ciliare) was normal (Fig.
To clarify anomalies of callose deposition, sets of pistils from sexual and aposporous buffelgrass were analyzed for callose fluorescence and reanalyzed using interference contrast microscopy.
Megasporogenesis in aposporous Kentucky bluegrass was disturbed more frequently and in a more variable manner than in the other aposporous species.
Callose was restricted to the wall of the meiocyte closest to the micropyle in 32% of Kentucky bluegrass pistils, which is similar to the frequency observed in aposporous P.
The frequently observed limitation of callose to the wall of the meiocyte closest to the micropyle in Kentucky bluegrass is correlated with early aposporous embryo sac initiation (Naumova et al., 1993).
Subsamples of 40 (10 sexual, 30 aposporous) and 30 (10 sexual, 20 aposporous) progeny from the B-2s x B-12-9 and B-2s x Higgins crosses, respectively, were screened for molecular markers by bulk segregant analysis (Michelmore et al., 1991).
Data were analyzed by the "[F.sub.2] backcross" option and presence of marker and aposporous phenotype were assigned to "A" = heterozygous dominant, and absence of marker and sexual phenotype were assigned to "H" = homozygous recessive.
The cross B-2s x Higgins yielded 15 sexual and 38 aposporous progeny (aaaa x AAaa, 3: 11 expected, [chi square] = 1.49, P = 0.22).
In our material, a C04-600 marker was expressed by the sexual parent and both aposporous parents.
Genomic DNAs of some individuals not included in the bulks were also examined: three sexual and five aposporous genotypes from the Higgins cross, and two sexual and two aposporous genotypes from the B-12-9 cross.
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