Mechanistically based in vitro tests for ocular irritation likely will depend on a) well-characterized ocular cellular models, b) assays
that measure biochemical end points of cellular injury, and c) a database of human responses.
The matching ready-to-use and validated SYBR(R) Green-based qRT-PCR assay
portfolio is supplemented by QIAGEN's QuantiTect(R) Primer Assays
for real-time RT-PCR providing specificity and sensitivity comparable to sequence-specific probe-based assays
Serum samples were assayed
in duplicate, and positive results were confirmed in separate assays
A simple and reliable method for estimation of protein content in latex called the Latex dotMetric protein assay
has been developed.
Shelby, is that while subsequent assays
pick up some carcinogens missed by the first test, they also contribute additional false positives and false negatives.
FMF is widely respected globally and has established a standard for first trimester screening which embraces the role of clinicians, laboratories and the assays
that are used.
For intracellular pathogens, the antibody titers and neutralization assays
represent routine immunologic tests that provide results after several weeks of infection.
Additionally, competitive receptor binding assays
using radiolabeled brevetoxins have shown promise (25).
The New York City Department of Health protocol reports a sample as positive only if it has all the following phenotypes: nonmotile, penicillin sensitive, [gamma]-phage positive, and positive by both cell wall and CAP-DFA assays
have been used to monitor the presence of brevetoxin activity predominantly in shellfish.