influenzae in most laboratories, although this technique is prone to misinterpretation because of nonspecific agglutination, cross-reactions, or autoagglutination
. The transition from slide-agglutination to PCR-based methods that detect capsule locus genes, such as bexA or bexB, has substantially improved the accuracy of serotyping results.
In the blood smear it is common to observe spherocytosis, anisopoikilocytosis, fragmented red cells, basophilic stippling, polychromatophilia, autoagglutination
, and nucleated erythrocytes [1, 4].
The antigen was tested for autoagglutination
and self-flocculation [17, 18]; flawless antigen was used in the study.
was not seen when VWF antibody-latex reagent alone was rotated for three minutes.
Platelet induced autoagglutination
of Plasmodium falciparum-infected red blood cells and disease severity in Thailand.
Because of these regional factors, the precise cutoffs and rules that proved most efficient in our laboratory may not be the optimal rules to apply in laboratories working in other regions of the globe, (3) Samples were collected consecutively in a period of 2 months, so some uncommon positive findings were not observed during the study, such as malaria, RBC autoagglutination
, and rouleaux formation.
Sequestration can be increased when adherent infected erythrocytes bind to other infected erythrocytes (autoagglutination
) or non-infected erythrocytes (resetting) or use platelets to bind to other infected erythrocytes (platelet-mediated clumping).
Procedure note: Because of nonspecific autoagglutination
, appropriate positive and negative controls are always included in the 0157 and H7 assays.
If the specimens have inadvertently cooled, warming to 37 C for at least 10 minutes will cause the cold antibody to elute back from the cells into the serum and the autoagglutination
will disperse completely in most cases.