After 14 days, the plates were returned to the laboratory and the number of autozooids and bifurcations counted for each colony.
The number of autozooids was counted for replicate boxes 1-15 on day 13 and for boxes 16-30 on day 14.
Larval swimming duration and colony orientation significantly affected growth as measured by the number of autozooids and bifurcations in a colony ([ILLUSTRATION FOR FIGURE 3A, B OMITTED]; Table I).
On average, 24-h colonies had half as many autozooids, bifurcations, and brood chambers as did 1-h colonies over the same time of development.
Because 24-h colonies initially grow more slowly, they will always have fewer autozooids, bifurcations, and brood chambers at a given point in time and, assuming similar growth potentials, they will never "catch up" to their 1-h counterparts.
silt) is inversely related to the number of autozooids actively feeding (Best and Thorpe, 1996).
Five sea pansies were anesthetized, their autozooid polyps were excised, and the polyp tissues were digested in the papain solution as described above.
Sea pansy colonies were anesthetized as described above and autozooid polyps, as well as rat heart tissues, were finely minced.
Small pieces of colonial tissue (rachis) bearing one autozooid polyp and several siphonozooid polyps (also containing photocytes) were excised from unanesthetized colonies and placed individually in assay vials containing 150 [[micro]liter] of ASW.
In both autozooid and siphonozooid polyps, photocytes are clustered in specific locations of the endodermal layer where cells filled with highly refringent granules (presumably acting as diffuse reflectors for the light emission) predominate (Anctil et al.
Specific Cx43-like immunoreactivity was present in all tissue layers of autozooid polyps but was more prevalent in the luminescent endoderm and in the mesogleal nerve net.