auxotrophic mutant


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auxotrophic mutant

[¦ȯk·sə¦trä·fik ′myüt·ənt]
(genetics)
An organism that requires a specific exogenous growth factor, such as an amino acid, for its growth.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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The yield in gram per gram dry cell weight g/gdcw by triple auxotrophic mutant GHA10 was Out of 8 different auxotrophs studied for L- lysine production, the triple auxotrophic mutant of glutamate-homoserine-alanine (MRLH-GHA10) was found to be the most potent mutant for L-lysine production.MRLH-GHA10 showed 38.4 g/L L- lysine on 4th day in FM1 medium in stirred tank fermentation at 30 C (pH 7.5, aeration 1.5 vvm, agitation 500 rpm).
Nutritional and Mutational Aspects of Lysine Production by Corynebacterium glutamicum Auxotrophs
Microorganism: Micrococcus glutamicus [AB.sub.100], a biotin requiring auxotrophic mutant, derived from a regulatory mutant Micrococcus glutamicus [AB.sub.1] by induced mutation [16].
Selection of suitable maintenance medium for the mutant Micrococcus glutamicus [AB.sub.100] as a L-glutamic acid producer
So, the use of auxotrophic mutants can prevent the reproduction of such organisms in the environment.
Lactococcus lactis: A New Strategy for Vaccination
Auxotrophic mutants are available in Drosophila (e.g., ade2; Johnstone et al., 1985), but they are hard to visualize and require special minimal media for selection to operate.
When a fly has to fly to reproduce: selection against conditional recessive lethals in Drosophila
Intracellular multiplication and virulence of Shigella flexneri auxotrophic mutants. Infect Immun 66:549-557 (1998).
Human Cell Exposure Assays of Bacillus thuringiensis Commercial Insecticides: Production of Bacillus cereus-Like Cytolytic Effects from Outgrowth of Spores

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