The yield in gram per gram dry cell weight g/gdcw by triple auxotrophic mutant
GHA10 was Out of 8 different auxotrophs studied for L- lysine production, the triple auxotrophic mutant
of glutamate-homoserine-alanine (MRLH-GHA10) was found to be the most potent mutant for L-lysine production.MRLH-GHA10 showed 38.4 g/L L- lysine on 4th day in FM1 medium in stirred tank fermentation at 30 C (pH 7.5, aeration 1.5 vvm, agitation 500 rpm).
Microorganism: Micrococcus glutamicus [AB.sub.100], a biotin requiring auxotrophic mutant
, derived from a regulatory mutant Micrococcus glutamicus [AB.sub.1] by induced mutation .
So, the use of auxotrophic mutants
can prevent the reproduction of such organisms in the environment.
are available in Drosophila (e.g., ade2; Johnstone et al., 1985), but they are hard to visualize and require special minimal media for selection to operate.
Intracellular multiplication and virulence of Shigella flexneri auxotrophic mutants
. Infect Immun 66:549-557 (1998).