The yield in gram per gram dry cell weight g/gdcw by triple
auxotrophic mutant GHA10 was Out of 8 different auxotrophs studied for L- lysine production, the triple
auxotrophic mutant of glutamate-homoserine-alanine (MRLH-GHA10) was found to be the most potent mutant for L-lysine production.MRLH-GHA10 showed 38.4 g/L L- lysine on 4th day in FM1 medium in stirred tank fermentation at 30 C (pH 7.5, aeration 1.5 vvm, agitation 500 rpm).
Microorganism: Micrococcus glutamicus [AB.sub.100], a biotin requiring
auxotrophic mutant, derived from a regulatory mutant Micrococcus glutamicus [AB.sub.1] by induced mutation [16].
So, the use of
auxotrophic mutants can prevent the reproduction of such organisms in the environment.
Auxotrophic mutants are available in Drosophila (e.g., ade2; Johnstone et al., 1985), but they are hard to visualize and require special minimal media for selection to operate.
Intracellular multiplication and virulence of Shigella flexneri
auxotrophic mutants. Infect Immun 66:549-557 (1998).