Affinity interactions between biotin and avidin
were characterized by the association rate constant [k.sub.a], dissociation rate constant [k.sub.d], and equilibrium association constant [K.sub.eq], where A is the injected analyte, B is the immobilized ligand, and AB is the analyte-ligand complex.
NHS activated palmitic acid (PA) reacts with positively charged Avidin
. Various amount of PA-Avidin was added to PLGA NPs to quantify the surface density of Avidin
and DAU anchoring onto the surface of MNPs were monitored by FTIR.
The complex of biotinylated myosin and avidin
was readily formed, and the attachment site of avidin
on the myosin head was 13 nm away from the head-rod junction, indicating the location of the SH1.
50 ul of Avidin
HRP was added to each well and incubated at the 37 C for 30 minutes and washed with wash buffer.
Avidin/biotin block was carried out using avidin
D for 15 minutes, followed by biotin for 15 minutes.
The target-bound biotinylated peptides were detected using ABC (Avidin
Biotin Complex) and ABTS solutions as described above.
conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated.
Herein, the USCA were composed of the lipid encapsulated non gaseous, perfluorocarbon emulsion that utilizes a triphasic targeting approach based upon the interaction of avidin
and biotin molecules as shown in figure 3.
In the first method, a ruthenium tris(bipyridine)-labeled avidin
film and a ds-DNA film were assembled successively on a tin oxide nanoparticle film electrode.
Measurement of proportions of active and inactive forms of acetyl-CoA crboxylase The proportion of polymeric (active) and of the protomeric (inactive) forms of acetyl-CoA carboxylase in the nuclear-free and cytosolic fractions of lactating rat mammary gland were measured by preparing homogenates of tissue, as described above, in the absence (that is with both the active polymeric and the inactive protomeric forms present) or in the presence (that is with only the active polymeric form present) of 400 mg avidin
Biomedical researchers in Europe and North America explain the application of laboratory methodology that exploits the powerful interaction between the protein avidin
or its homolog, and the vitamin biotin and some of its homolog.