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Ab and monomeric HLA-A2 were conjugated by making use of the high affinity between avidin and biotin: avidinylated-Ab was incubated with biotinylated HLA-A2 at different ratios for 2 h prior to use in experiments.
The complex of biotinylated myosin and avidin was readily formed, and the attachment site of avidin on the myosin head was 13 nm away from the head-rod junction, indicating the location of the SH1.
Avidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated.
Herein, the USCA were composed of the lipid encapsulated non gaseous, perfluorocarbon emulsion that utilizes a triphasic targeting approach based upon the interaction of avidin and biotin molecules as shown in figure 3.
In the first method, a ruthenium tris(bipyridine)-labeled avidin film and a ds-DNA film were assembled successively on a tin oxide nanoparticle film electrode.
Measurement of proportions of active and inactive forms of acetyl-CoA crboxylase The proportion of polymeric (active) and of the protomeric (inactive) forms of acetyl-CoA carboxylase in the nuclear-free and cytosolic fractions of lactating rat mammary gland were measured by preparing homogenates of tissue, as described above, in the absence (that is with both the active polymeric and the inactive protomeric forms present) or in the presence (that is with only the active polymeric form present) of 400 mg avidin .
Biomedical researchers in Europe and North America explain the application of laboratory methodology that exploits the powerful interaction between the protein avidin or its homolog, and the vitamin biotin and some of its homolog.
Another question that often comes up in discussions about home-prepared canine diets has to do with the avidin in raw egg whites causing a biotin deficiency, but there is no need to worry.
Ark Therapeutics has developed Scavidin, a version of a naturally occurring molecule called avidin, which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then "shuttles" it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour.
Ark Therapeutics has developed Scavidin - a version of a naturally occurring molecule called avidin - which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then 'shuttles' it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour.
Avidin is a protein normally found in chicken egg whites and binds biotin (DeLange and Huang, 1971).
Avidin had already been identified and well studied in chickens.
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