The final bacterial pellet was suspended in minimal medium consisting of NaP[O.sub.4] buffer supplemented with 100 mM NaCl and 1% TSB to sustain bacterial luminescence as previously described (Abou Alaiwa et al.
As a control, we coincubated bacteria with CFA and observed no increase in bacterial luminescence (data not shown).
Decrease of 500 mL bacterial luminescence
in effect of 10 mL biocide measured by luminometer after 2 min.
anguillarum was transformed with the plasmid pRB28, which is composed of the regulatory region (merR) of the mer operon (but not merA, the genes for Hg(II) reduction to [Hg.sup.0]) and of lux genes coding for luciferase (luxAB) and fatty acid reductase (luxCDE), required for bacterial luminescence
. Hg(II) entering cells containing this plasmid would not be reduced (as it would in nature if the cells contained the entire mer operon), but will cause transcription of the lux genes.
The functional symbiotic organ is composed of several host tissues, including a core of branching epithelial crypts that houses the symbionts; a thick reflector that directs the bacterial luminescence ventrally; diverticula of the ink sac that dynamically control the intensity of
Specifically, a morphology associated with inoculation is replaced by a morphology that mediates the mature function of the symbiotic state, i.e., the controlled emission of bacterial luminescence into the host's environment.