The sunflower oil cake was supplemented with (in mg) soybean meal 250, calcium carbonate 75, potassium nitrate 100, barbital
20, and magnesium sulphate 1 with predetermined quantity of water.
Electrophoresis was performed at 95 Volts, for 35 min using barbital
buffer pH 8,6.
Over 2500 derivatives of barbituric acid have been synthesized after the triumph of barbital
and phenobarbital in epilepsy.
Alkaline phosphatase (marker for secretory vesicles/plasma membrane) was measured by hydrolysis of p-nitrophenyl phosphate, at pH 10.5, in a sodium barbital
The Beckman system used a 1% agarose gel, barbital
running buffer with a pH of 8.6, a run time of 37 minutes at 100 volts, and Coomassie blue stain.
These RIAs use 150 [micro]g 8-anilino-1-naphthalene sulfonic acid in each assay tube, and barbital
buffer to eliminate interference of serum binding proteins.
Intravenous diazepam achieved quicker sedation of patients, compared with rectal paraldehyde in one study, and there was no difference in sedation times between intramuscular diazepam and oral barbital
in another, said Dr.
Briefly, tissue samples were homogenized in 10 X vol of chilled 25 mmol [1.sup.-1] veronal buffer (25 mmol [1.sup.-1] barbital
buffer, 5 mmol [1.sup.-1] EDTA, 5 mmol [1.sup.-1] dithiothreitol, and 10 mmol [1.sup.-1] MgS[O.sub.4], pH 8) and centrifuged for 10 min at 1200 X g.
Serial dilutions of human CNP-22, BNP-32, and ANP-28 in barbital
buffer measured with the CNP RIA revealed cross-reactivity for BNP-32 in physiological concentrations (Fig.
Potentiation of barbital
hypnosis as an evaluation method for central nervous system depressant.
Supematants were pooled and evaporated to dryness under nitrogen, and desiccated samples were resuspended in 0.25 mL barbital
buffer containing 2.5 mg/mL anilino naphthalene sulfonic acid (to disrupt the coupling between thyroid hormones and serum proteins, including lipoproteins), 0.25 mL ethanol, and 1 mL chloroform.
Stock buffer was composed of 10 mmol/L sodium barbital
, 2 mmol/L potassium phosphate monobasic, and 383 mmol/L sodium chloride.