was found 3.13 times lower than non-hemolytic isolates, while it was 1.37 and 2 times higher than the isolates displaying alpha and alpha-beta hemolysis, respectively (Table II).
(5) Isolates presenting coccus morphology, Gram positive stain, beta hemolysis
, and producing catalase, coagulase, and DNAse were submitted to PCR for identification of S.
The observation of colonial, morphological and biochemical characters of Staphylococcus aureus revealed medium to large colonies, large, convex, white colonies with a narrow zone of beta hemolysis
on blood agar.
Only strain V1 exhibited beta hemolysis
: an obviously clear zone was observed around colonies of strain V1 on the sheep blood agar after the plate was incubated at 15[degrees]C for 48 h, demonstrating that hemoglobin in the clear zone could be completely degraded by strain V1.
Colonies will exhibit beta hemolysis
and need to be differentiated from other beta-hemolytic organisms.
The pathogenic organisms were identified by gross colony morphology and types of hemolysis (yellowish halo around the colony beta hemolysis
or turbid halo with greenish cast around colony alpha hemolysis) on culturemedia.