Jo Realising they had identified a process with huge commercial potential internationally the research team, led by Professor Jo Bradwell, created the Binding Site
as a commercial venture.
It is easy to grasp then how a mutation within a promoter, changing a TF binding site
, can lead to problems not through a change in the function of the mature gene product, but through variation in expression level of the product.
The binding site
prediction is also very important for target drug design.
Positions of the amino acids present in binding site
of the protein and tertiary structure representation of the binding site
were determined by FTsite server from Boston University, which is freely available online at http://ftsite.bu.edu/.
Conventional stepwise complex formation algorithm best fits the data matrix which included one strong binding site
and one weak binding site
(lg [K.sub.1-2] = [7.07 5.79]), lof([A.sub.3e]) [approximately equal to] 0.25%.
A binding site
is defined as a collection of the interacting atoms that bind to the same nucleotide molecule.
It contains tryptophan and tyrosine in the binding site
. The Y469 formed CH/pi stacking with Glc2 and W460 formed stacking with Glc4.
Bound DNA was purified and amplified by PCR with primers (forward 5'-TCCTGCCAAGTGTGTCAAAT-3' and reverse 5'GTGAACGCAGAAATACCAGAA-3') which amplified the region of the FGF21 promoter containing target Sp1-B binding site
. The PCR products then were separated on 2% agarose gels.
 reported that in patients with advanced liver disease, the reduction of binding capacity at albumin binding site
II that binds to aromatic carboxylic compounds is related to impaired liver function.
It is notable that a 200-bp region immediately upstream of the transcription start site contained highly conserved transcription factor-binding motifs, including the Oct4/Sox2 binding site
and Sp1 binding motifs.
Data from laboratories running Optilite show 40% faster processing of complex protein assays compared to other special protein analyzers on the market, Binding Site
Further investigation helped to elucidate the stereochemical specificity of the carbohydrate binding site
to the bacterial ligand.