Victor Nadler," Hippocampal mossy fiber sprouting and synapse formation after status epilepticus in rats: visualization after retrograde transport of biocytin
," Journal of Comparative Neurology, vol.
During whole-cell patch clamp electrophysiological recordings, some neurons were filled with biocytin
so their morphology could be recovered.
Zymosan administration 3 days prior to optic nerve injury highly stimulated RGC axonal regeneration, assessed at 7 days after injury via anterograde biocytin
tracing as described previously [113, 114].
was included in the pipette solution at 0.5-1 mg/ml to retrospectively identify recorded cells.
(MPB) was purchased from Invitrogen (USA).
To measure this correlation, sensory neurons were injected with biocytin
to label their sprouting after lesioning.
In addition, catabolism of biotin leads to formation of many metabolites such as bisnorbiotin and biocytin
. We measured the biotin concentration in the patient's specimen using a microbial growth assay that underestimates biotinylated molecules (11), suggesting that the actual concentration of biotinylated molecules present may be substantially higher than 4.8 [micro]g/L.