Following this colonies from the colony count section of the CLED agar (Figure-1) were sub-cultured onto a Chromogenic agar (CHROMagar) and Sabouraud dextrose agar (SDA) biplate (Oxoid UK) to obtain a presumptive identification of the Candida species and also to determine if the urine sample had more than one species of Candida.
The original urine specimen was not used for direct culture onto the CHROMagar/SDA biplate because by the time yeast colonies are visible on the CLED agar the urine specimen would have already been kept for more than 24 hours and hence there will be bacterial overgrowth in the urine specimen which may overwhelm the yeast when subculture onto CHROMagar/SDA is attempted.
Comparison of CPS ID 3 and CHROMagar Orientation chromogenic agars with standard biplate
technique for culture of clinical urine samples.
During the hardening of the film, due to the appearance of shrinkage and adhesion forces, the new biplate
is being bent.
HUrBi is formulated to isolate Gram-positive organisms on the left side of the biplate
and Gram-negative organisms on the right side.
Samples were plated on biplate
blood agar, MacConkey agar, and Hectone-enteric and SalmonellalShigella-specific agar for Salmonella species, and then were incubated at 36[degrees]-37[degrees]C for 96 hours.
The laboratory has used broth (BACTEC 460) and solid media (Mitchison/Middlebrook biplate
, Remel, Lenexa, Kan) for all specimens processed from 1988 to the present.
The MGIT broth was subcultured to a Middlebrook 7H10/S7H11 agar biplate
and incubated at 37[degrees]C in 5% C[O.
4 Eliminating his one plate and using a blood/MacConkey biplate
instead of the two other whole plates saved nearly $5,500.
The most common culprits: stock organisms that failed to grow, contaminated stocks, and organisms transferred to the wrong side of a biplate
under a "pseudonym.
Suspected colonies were inoculated onto biplates
containing buffered charcoal yeast extract medium with and without L-cysteine.
BBL Sheep Blood Agar-Chocolate Agar biplates
(SBA-CHOC) were used for the wine samples and the fingertip samples.