that served as a positive control were incubated in DNase I solutions (5 U/50 [micro]L).
Total mRNA from the single blastocysts
was isolated using the Dynabeads mRNA Direct Kit (Dynal Asa, Oslo, Norway).
For differential staining of inner cell mass (ICM) and TE cell compartments, the blastocysts
which had been stained by PI were incubated in Triton X-100 prepared in the base medium for 20 seconds.
Embryologists are increasingly realizing that chromosomally abnormal embryos can appear to be developing properly and look quite normal at the blastocyst
However, the New York Times reported Kass wrote in an e-mail: "The age of human cloning has apparently arrived: today, cloned blastocysts
for research, tomorrow cloned blastocysts
Once Hwang and his colleagues optimized experimental conditions, about 25 percent of the eggs reached the blastocyst
14]C(U)-glucose, accumulation of ammonia after IVM, IVF and IVC and number of cells within blastocysts
As the time approaches for the blastocysts
to be implanted, the mother bear's blood contains more and more progesterone.
Because the cells grow and divide indefinitely in culture, very few blastocysts
would be needed.
They suck the nucleus out of a blastocyst
cell with a micro-pipette while holding it by suction under a microscope, then use this nucleus to replace that of an unfertilized ovum.
In May 2007, CCRM commenced this innovative, world first clinical study, which screens for all 23 pairs of chromosomes on a few cells removed from the blastocyst
using a technique called comparative genomic hybridization (CGH).
Therefore, the present study evaluated whether these blastocysts
could be used to derive pESCs and assessed the effects of deriving pES-like cells from IVF-aggregated blastocysts