Although our success percentage of 4% seems to be relatively low, it is quite adecuate if one considers that we are dealing with deer where the rate of development up to the blastocyte
stage is less than 10% in in vitro produced embryos (Locatelly & Mermillod).
and hatched blastocyst development rate was then monitored following in vitro fertilization.
Embryonic stem cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette were injected into C57BL/6J blastocytes
, and the resulting males were bred to 129/Sv females.
Expression of Nanog has been observed in both the inner cell mass (ICM) and trophectoderm of bovine blastocytes
; however, its expression is restricted to the ICM in human and mouse blastocytes
(Chambers et al., 2003; Mitsui et al., 2003; Kuijk et al., 2008; Munoz et al., 2008).
Embryonic stem cells, including totipotent stem cells (produced from fusion of egg and sperm), and pluripotent stem cells (5-14 day old blastocytes
) have a strong potency of cell reproducing and potentially highly teratogenic.