The genetic data from single
blastomere may not be representative of the one for the whole embryo.
Perfecting assisted reproduction technique holds potential benefits for determining sex of embryos by
blastomeres sexing, supporting the conservation efforts of avian species, and benefiting research areas, such as genetic and biopharmaceutical research.
Lanza, "Human embryonic stem cell lines derived from single
blastomeres," Nature, vol.
3C and 4D) and was later absorbed into one of the two cells of the first cleavage to form large and small
blastomeres. Cytokinesis was observed at times synchronous to the absorption of the PL.
It is based on PCR amplification (most often) of the entire genome presented from only one cell, which makes this method equally efficient for polar body,
blastomere or trophectoderm (TE) cell analysis [1].
Many technical issues are associated with WGA, from the type of cells used (
blastomere versus trophectoderm [7]), to the number of cells used for the WGA step [6], to the quantity and quality of DNA, all of which can impact ADO and FA rates.
The increase of cell segmentation enhances the degree of compaction between
blastomeres and marks the end of the ball stage (Wolpert et al., 2000), also referred to as morula and early blastula (Andrade-Talmelli et al., 2001; Reynalte-Tataje et al., 2001; Romagosa et al., 2001; Anjos and Anjos, 2006; Ninhaus-Silveira et al., 2006; Faustino et al., 2007; Paes, 2008; Nakaghi et al., 2013).
Transfer of mtDNA even in the form of
blastomere nuclei (the technique which has been scrutinised the most by ethicists) is not 'reproductive cloning of a human being'.
Each
blastomere cell was transferred into a PCR tube containing 3.5 [micro]L of PBS.
(1) After filtering the gray image, the
blastomere edges were obtained using the canny operator; however, the image may contain some noise and fragments, which should be deleted in the following steps.
We would certainly hope that our patented, proprietary 'embryo-safe' single-cell
blastomere technique would be a part of any such conversation.