Hair sheep blood, citrated or defibrinated, fulfills all requirements of blood agar
for diagnostic microbiology laboratory tests.
Each of the isolates showed grey, viscous, mucoid, translucent and non-hemolytic colonies on blood agar
after 24 hours incubation at 37oC but failed to grow on MacConkey's agar.
FG discs were inoculated into the pure blood agars
(Biomerieux) (Group 1) and bacteria-cultured agars (Groups 4a, 4b, 5a, and 5b) (immediately after bacteria cultivation).
The four chromogenic media were compared with blood agar
and evaluated in terms of sensitivity, specificity, ease of use, and cost.
The haemolytic pattern in 10 per cent sheep blood agar
showed narrow zone of haemolysis, typical for the STEC.
In this study, more over all types of blood agar
(human, sheep and chicken blood) supported the growth of Acanthamoeba (Table 3).
(nutrient agar plus 5% sheep red blood cells) and chocolate agar (nutrient agar plus powdered hemoglobin) are examples of solid-enriched media that are routinely used in the laboratories (Engelkirk and Burton 2007).
They were submitted to the simplified identification key, which consisted of hemolysis detection in ovine blood agar
, trehalose, manitol and maltose fermentation, as well as acetoin production (VP), selected on the basis of the identification keys proposed by Holt et al.
The swabs were inoculated in Columbia sheep blood agar
and selective enrichment broth.
A single colony of each isolate was streaked across blood agar
plates using sterile inoculating wire loop.
Data from the blood agar
indicated the mean number of CFU for the control toothbrush was 3.
Pre-enrichment in Alkaline Peptone Water allows Vibrio species to grow to detectable levels prior to plating on a suitable culture medium, such as TCBS (CM0333) or Blood Agar