GRD E strips were applied on the Mueller-Hinton blood agar
plates and incubated at 35AdegC for up to 48 hours.
Each of the isolates showed grey, viscous, mucoid, translucent and non-hemolytic colonies on blood agar
after 24 hours incubation at 37oC but failed to grow on MacConkey's agar.
Preparation of media: HiChrome UTI agar MacConkey agar and base for Blood agar
media were obtained as a dehydrated powder from
Effect of blood agar
from different animal blood on growth rates and morphology of common pathogenic bacteria.
The isolates did not cause haemolysis on blood agar
(BA) and failed to grow on MacConkey agar (MCA).
To check for bacterial growth, samples of the broth were then cultured on blood agar
at 37[degrees]C for 18 h and a biochemical test was used to identify the observed microorganisms.
anthracis Colony on blood agar
Mucoid and orange Gray-white to white Spore production - + (central) Motility + - Hemolysis on blood agar
- - Penicillin susceptibility + + Catalase production + + Indole production - - Growth at 4[degrees]C + - Anaerobic growth + + Cutaneous infection Ulcer, black eschar, Eschar, malignant blister pustule Other infections None reported Intestinal anthrax, pulmonary anthrax, meningitis * +, present; -, absent.
Briefly, the sample was inoculated onto 5% sheep blood agar
aerobically and incubated at 37[degrees]C for 48 hours.
and SDA agar were incubated at 37[degrees]C.
Spinal fluid was plated on 5% sheep blood agar
(BBL) and chocolate blood agar
(BBL) and was inoculated in to thioglycolate broth.
The bacterial samples had been collected deeply from infected tissues using sterile swabs and inoculated immediately into blood agar
plates and MacConkey plates and incubated for 24 hours at 37[degrees]C.
Microbiological cultures of the liver biopsy sample were performed on 5% sheep blood agar
and Sabouraud agar medium supplemented with chloramphenicol.