Four different chromogenic agar culture plates: CHROMagar[TM] StrepB, ChromID, Oxoid Granada, and StrepB Select, were compared with 5% Columbia blood agar
and routine laboratory detection.
In our study we took steps to ensure optimal bacterial detection, including the use of an enrichment broth in addition to blood agar
, and preliminary testing to exclude the possibility of an inhibitory effect of the fentanyl solution on bacterial growth.
A white filamentous mold grew after the sample was incubated for 2 days on Sabouraud agar at 30[degrees]C and 5% sheep blood agar
medium at 37[degrees]C.
An inoculation control plate (Columbia sheep blood agar
, Fort Richard Laboratories, Auckland) was inoculated between each antibiotic series and after all plates had been inoculated.
was prepared by allowing sterilized nutrient agar cool to 45[degrees]C and 5% sterile defibrinated sheep blood was aseptically added, mixed and poured into Petri dishes to set.
Subcultures were done on blood agar
, MacConkey agar and Salmonella-Shigella agar and incubated aerobically at 37[degrees]C for 18 to 24 h.
Strain 501 grew on blood agar
and formed mucoid colonies.
Samples for aerobic culture were plated on Columbia blood agar
and MacConkey agar (Remel, Lenexa, KS, USA) and incubated a minimum of 48 hours at 37[degrees]C with 5% carbon dioxide.
The broth was then streaked onto sheep's blood agar
plates and incubated once again.
Tryptone yeast broth and basal feather or snake skin medium were used to detect specific keratinase activity and pigment production, Mueller-Hinton plates for microbial interactions and antibiotic production, blood agar
and basal medium for hemolytic action and carbon utilization by Streptomyces.
Oxoid has produced a safer alternative to the original Butzler formulation for selective isolation of thermotolerant Campylobacter species from foods and animal feedstuffs from Columbia Blood Agar
Bacillus anthracis is nonmotile in a direct wet mount prepared from growth on the initial culture plate, nonhemolytic on sheep blood agar
, and any attempt to move the colony with an inoculating loop will result in growth standing up like a stiffly beaten egg white.