blotting

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blotting

[′bläd·iŋ]
(cell molecular)
The transfer of electrophoretically separated polypeptides onto a solid support medium, such as nitrocellulose paper or a nylon membrane.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
B, Expression levels of migration-associated proteins were assessed by western blot analysis. C, Cell invasion was detected with the transwell invasion assay.
The identity of purified IFN[alpha]2-T[alpha]1 was confirmed by immuno blot analysis with mouse anti-interferon [alpha]-2 and mouse anti-thymosin [alpha]-1 antibodies respectively (Fig.
We performed Western blot analysis of [PrP.sup.Sc] from brain homogenates as previously described (16) and performed preliminary diagnosis with a final concentration of PK at 50 [micro]g/mL.
(b) The total protein was extracted from the lung tissue of mice for Western blot analysis. (c, d, e) The expression of COL I, COL III, and [alpha]-SMA in the lung tissue of mice was determined by Western blot analysis, with GAPDH as an internal reference (* P < 0.05 versus control group, (#) P < 0.05 versus Ova-treated group).
Western Blot Analysis. We performed western blot analysis to investigate the expression levels of DDC and VMAT-2 in the lesioned striatum.
ASCs were further characterized for the expression of mesenchymal and epithelial markers (vimentin and cytokeratin 14, resp.) by both immunofluorescence and Western blot analysis, to confirm the absence of epithelial contaminants.
(a) Representative and statistical results of Western blot analysis showed the membrane translocation of cPKC[beta]II in the cortex, hippocampus, and striatum at 2 weeks after STZ injection.
(a) Representative Western blot analysis and real-time PCR showed VAPA was knocked down by shRNA transfection.
Western Blot Analysis. RPE cells were prepared with protein extraction and protease inhibitor kits (Pierce, Rockford, IL, USA).
Western blot analysis of whole cell lysates using the Sema3A antibody showed a protein band of 106 kDa.
The former assessment can be made by immunohistochemical analysis and the latter by proteinase K treatment followed by Western blot analysis for PrP or visualization of [PrP.sup.Sc] fibrils.
The homogenate was centrifuged at 12,000 g for 20 min at 4 C and the resulting supernatant was used for dot blot analysis. For Dot blot analysis, Oak1 antibodies were employed to detect the presence of Oak1 protein expression following the method described by Lai et al.