B, Expression levels of migration-associated proteins were assessed by western blot analysis
. C, Cell invasion was detected with the transwell invasion assay.
The identity of purified IFN[alpha]2-T[alpha]1 was confirmed by immuno blot analysis
with mouse anti-interferon [alpha]-2 and mouse anti-thymosin [alpha]-1 antibodies respectively (Fig.
We performed Western blot analysis
of [PrP.sup.Sc] from brain homogenates as previously described (16) and performed preliminary diagnosis with a final concentration of PK at 50 [micro]g/mL.
(b) The total protein was extracted from the lung tissue of mice for Western blot analysis
. (c, d, e) The expression of COL I, COL III, and [alpha]-SMA in the lung tissue of mice was determined by Western blot analysis
, with GAPDH as an internal reference (* P < 0.05 versus control group, (#) P < 0.05 versus Ova-treated group).
Western Blot Analysis
. We performed western blot analysis
to investigate the expression levels of DDC and VMAT-2 in the lesioned striatum.
ASCs were further characterized for the expression of mesenchymal and epithelial markers (vimentin and cytokeratin 14, resp.) by both immunofluorescence and Western blot analysis
, to confirm the absence of epithelial contaminants.
(a) Representative and statistical results of Western blot analysis
showed the membrane translocation of cPKC[beta]II in the cortex, hippocampus, and striatum at 2 weeks after STZ injection.
(a) Representative Western blot analysis
and real-time PCR showed VAPA was knocked down by shRNA transfection.
Western Blot Analysis
. RPE cells were prepared with protein extraction and protease inhibitor kits (Pierce, Rockford, IL, USA).
Western blot analysis
of whole cell lysates using the Sema3A antibody showed a protein band of 106 kDa.
The former assessment can be made by immunohistochemical analysis and the latter by proteinase K treatment followed by Western blot analysis
for PrP or visualization of [PrP.sup.Sc] fibrils.
The homogenate was centrifuged at 12,000 g for 20 min at 4 C and the resulting supernatant was used for dot blot analysis
. For Dot blot analysis
, Oak1 antibodies were employed to detect the presence of Oak1 protein expression following the method described by Lai et al.