The researchers developed a novel algorithm that can recover the phase information from a stack of bright-field
images taken by a classical microscope.
RNAscope is compatible with routine formalin-fixed, paraffin-embedded tissue specimens and can use either conventional chromogenic dyes for bright-field
microscopy or fluorescent dyes for multiplex analysis.
The eyepieces are suited for use with standard achromatic objectives and upright, bright-field
As such, our group has developed and previously published data on a second-generation automated bright-field
dualcolor in situ hybridization assay for MDM2.
Z1 is a whole slide scanner for bright-field
and fluorescence samples that is utilized to record virtual slides of tissue sections in research and clinical pathology labs around the world.
This system consists of two technologies, such as bright-field
inspection and dark-field inspection.
The Opterra II also features a sub-10% field illumination uniformity; options for bypass capability for wide-field, bright-field
, and TIRF imaging; a high-speed 10-position filter wheel; as well as photo-activation, spectral imaging and un-mixing capabilities.
Thus, one method to obtain a 3-D color image is to do a two-scan measurement: one scan with an interferometric objective delivering 3-D measurement data based on finding a best focus position for each pixel, and a second scan with a bright-field
objective during which imaging information is collected only at the previously predetermined best-focus positions and then overlaid on the 3-D measurement data.
The technology is compatible with FFPE specimens, conventional chromogenic and fluorescent dyes, and is suitable for bright-field
microscopy and multiplex analysis.
In this activity, students will (1) learn the applications and use of ImageJ, (2) learn to measure cell area from a bright-field
image and compute data to reach conclusions, and (3) explore on their own the use of the cell-counter plug-in for ImageJ.
Particle-size distributions were obtained by manual measurement of particle diameters from bright-field
In a simpler and more straightforward alternative, the problem of autofluorescence can be avoided altogether by basing detection on enzymatic development of a substrate and visualizing the locations of the reaction products with bright-field