In the DPPH radical-scavenging assay, Table 2 shows the concentrations of butylated hydroxyanisole
(BHA) and the Thai herb extracts, at which the DPPH radicals were scavenged by 50 % ([IC.
N-(1-naphthyl) ethylene Diamine Dihydrochloride (NEDD), Sodium nitoprusside, sulphanilic acid, sodium chloride (NaCl), Ferric Chloride (FeCl3), disodium hydrogen orthophosphate, potassium dihydrogen phosphate, 2,2'-Azino- bis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS), butylated hydroxyanisole
(BHA), butylated hydroxytoluene (BHT), 1,10-Phenanthroline and Folin-Ciocalteu Reagent.
(E320) has caused birth defects in laboratory animals.
Cholesterol, D-mannitol, and butylated hydroxyanisole
were from Sigma Chemical Co.
In rats, hyperplastic and neoplastic changes in the forestomach may result from the chronic administration of butylated hydroxyanisole
The chlorogenic acid data encouraged the scientists to also test butylated hydroxyanisole
, better known as BHA.
The additives were sodium ascorbate, SA sodium tripolyphosphate, STPP; butylated hydroxyanisole
, BHA; and t-butylhydroquinone, TBHQ.
In the meat industry, mainly synthetic antioxidants are used to slow the lipid oxidations such as butylated hydroxytoluene (BHT), butylated hydroxyanisole
, tertiary butyl hydroquinone, and propyl gallate.
Synthetic antioxidants such as butylated hydroxyanisole
(BHA) and butylated hydroxytoluene (BHT) are in use but these are prohibited due to their in-vivo carcinogenic effects .
The antioxidant activity of anthocyanins from cherries was comparable to the commercial antioxidants, tert-butylhydroquinone, butylated hydroxytoluene and butylated hydroxyanisole
, and superior to vitamin E, at a test concentration of 125 microg/ml.
The experimental data showed that ramalin was five times more potent than commercial butylated hydroxyanisole
(BHA) in scavenging l-diphenyl-2-picryl-hydazil (DPPH) free radicals, 27 times more potent in scavenging 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid free radicals (ABTS*+) than the vitamin E analogue, trolox, and 2.
All reagents include Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl, gallic acid, sodium carbonate, vitamin E, butylated hydroxyanisole
, EDTA and all solvents (HPLC grade) were obtained from Fluka (Switzerland).