There is evidence that
butylated hydroxytoluene, as a phenolic anti-oxidant, acted as a membrane lipid perturbant which prevented or substantially reduced the permeability changes of sperm plasma membrane when the cell was cold-shocked (Hammerstedt et al., 1976).
The antioxidant activity was expressed relative to that of
butylated hydroxytoluene (BHT).
Toxicology and biochemistry of butylated hydroxyanisole and
butylated hydroxytoluene. J.
These compounds demonstrated diverse in vitro DPPH radical scavenging activities with IC50 values ranging between 55.2 +- 1.2-149.6 +- 1.7 uM, as compared to standard BHT (
butylated hydroxytoluene) (IC50 = 128.8 +- 2.1 uM).
It is an in-vitro study to determine the free radical scavenging activity of Mucuna pruriens by DPPH assay in comparison with a standard compound (BHT-
Butylated Hydroxytoluene) using spectrophotometer.
Folin-Ciocalteu reagent, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH),
butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and L-ascorbic acid (L-AA) were purchased from Sigma Chemical Co.
Butylated hydroxytoluene (BHT) was listed as the chemical found in the breath print of AD and PD patients [15].
These results were compared using butylated hydroxyanisole (BHA),
butylated hydroxytoluene (BHT), and [alpha]-tocopherol (TOC) as standard antioxidant compounds.
Differential effects of
butylated hydroxytoluene analogs on bull sperm subjected to cold-induced membrane stress.
The commercial known antioxidants,
butylated hydroxytoluene (BHT), Vitamin E and ascorbic acid were used as positive control for comparison.
In the meat industry, mainly synthetic antioxidants are used to slow the lipid oxidations such as
butylated hydroxytoluene (BHT), butylated hydroxyanisole, tertiary butyl hydroquinone, and propyl gallate.
Additionally, green tea (Camila sensis) holds greater antioxidant protection than the ordinary polyphenols in antioxidant vitamins such as C and E (Tedeschi et al., 2004), also its antioxidative activity is stronger than butylated hydroxyanisole,
butylated hydroxytoluene and DL-alpha-tocopherol.
