This leaves a molecule called c-Rel
free to jump in and promote normal T cell proliferation.
Empleando el metodo de doble delta Ct, se calculo la dosis genica para c- MYC, MYCN, MYCL, EGFR, AKT-2, ERBB2, PIK3CA y c-REL para un panel de lineas celulares de origen tumoral pulmonar.
c-Rel es un importante factor de transcripcion de la familia cRel/NF-kB, participa activamente en la expresion de genes involucrados en el desarrollo, proliferacion y supervivencia de celulas linfoides (Houldsworth et al., 1996).
The Rel/NF-[kappa]B family of transcription factors is comprised of 5 members, NF-[kappa]B1, NF-[kappa]B2, RelA, RelB, and c-Rel. Homo- and heterodimers of Rel/NF-[kappa]B proteins exist as latent complexes bound to I[kappa]B (inhibitor of [kappa]B) proteins in the cytosol.
Primarily the NF-[kappa]B family member, c-Rel, has been demonstrated to be important in Foxp3 induction during tTreg development.
Only NF-[kappa]B/c-Rel was found to be modulated by [DELTA]Np63[alpha] under these conditions, with nuclear accumulation of phosphorylated c-Rel but none of the other NF[kappa]B subunits enhanced in the presence of overexpressed [DELTA]Np63[alpha].
characterized a dynamic mechanism whereby c-Rel, [DELTA]Np63[alpha], and TAp73, which are coexpressed in the nuclei of a subset of HNSCC cell lines, control expression on binding sites including [p21.sup.WAF1], Noxa, and Puma .
Five members of the NFkB family have been identified: NFkB1 (p50/pl05), NFkB2 (p52/pl00), RelA (p65), RelB, and c-Rel
. They share a highly conserved Rel homology domain at the N-terminal end that is responsible for specific DNA binding, dimerization, and interaction with IkB.
These four studies do not describe basic technology, which is a topic that is already covered well elsewhere, but give leading-edge applications and research, including the use of microarrays in studies of gene regulation in yeast, transcription regulatory networks in the yeast cell cycle, transcriptional regulatory networks from yeasts to humans, the identification of mammalian E2F networks using DNA microarray hybridization analyses, the nuclear factor kB gene regulatory network, transcriptional networks in mammalian gene regulation, deciphering regulatory networks controlled by the NF-kB transcription factor c-Rel
in T-cells, transcriptional profiling of the hepatic growth response, transcriptional responses to DNA damage, and genetic genomics and Bayesian networks.
Antibodies against p65 (H-286), p50 (nuclear localization signal), c-Rel
(N466), and p52 (447) (donated by N.
In addition, for the supershift assay, the antibodies directed to the NF-[kappa]B family proteins p50 (SC-1190X), p65 (SC-109X), c-Rel
(SC-6955X), Rel-B (SC-226X), and p52 (SC-7386X; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were preincubated with nuclear extracts for 10 min on ice before the addition of the radiolabeled probe.
These include NF-[kappa]B1 (p50/p105), NF-[kappa]B2 (p52/p100), p65 (ReIA), Re1B, and c-Rel