Five members of the NFkB family have been identified: NFkB1 (p50/pl05), NFkB2 (p52/pl00), RelA (p65), RelB, and c-Rel.
Dependence on nuclear abundance of Rel A, NF-kappaB1, and c-Rel transcription factors.
These four studies do not describe basic technology, which is a topic that is already covered well elsewhere, but give leading-edge applications and research, including the use of microarrays in studies of gene regulation in yeast, transcription regulatory networks in the yeast cell cycle, transcriptional regulatory networks from yeasts to humans, the identification of mammalian E2F networks using DNA microarray hybridization analyses, the nuclear factor kB gene regulatory network, transcriptional networks in mammalian gene regulation, deciphering regulatory networks controlled by the NF-kB transcription factor c-Rel
in T-cells, transcriptional profiling of the hepatic growth response, transcriptional responses to DNA damage, and genetic genomics and Bayesian networks.
Antibodies against p65 (H-286), p50 (nuclear localization signal), c-Rel (N466), and p52 (447) (donated by N.
No supershifting occurred, so band elimination was the indicator for the presence of c-Rel in the HMEC-1 extracts (Fig.
In addition, for the supershift assay, the antibodies directed to the NF-[kappa]B family proteins p50 (SC-1190X), p65 (SC-109X), c-Rel (SC-6955X), Rel-B (SC-226X), and p52 (SC-7386X; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were preincubated with nuclear extracts for 10 min on ice before the addition of the radiolabeled probe.
The NF-[kappa]B subunits were examined using Abs specific for p65, p50, p52, Rel-B, and c-Rel in supershift assays.
These include NF-[kappa]B1 (p50/p105), NF-[kappa]B2 (p52/p100), p65 (ReIA), Re1B, and c-Rel