Characterization of agonist stimulation of cAMP-dependent protein kinase
and G protein-coupled receptor kinase phosphorylation of the beta2-adrenergic receptor using phosphoserine-specific antibodies.
PKA, also called cAMP-dependent protein kinase
A, is organized as tetramers comprising two catalytic and two regulatory subunits.
For positive control along with reaction mixture dilutions (using PKA dilution buffer- 380 mM K3PO4 andmM DTT) of cAMP-dependent protein kinase
in variable concentrations (0.
1992) Catalytic subunits of Aplysia neuronal cAMP-dependent protein kinase
with two different N termini.
We examined Boa's ability to activate different downstream signaling transduction pathways (kinases) by retreating GC-1 cells with the PKG inhibitor KT5823, the ERK inhibitor PD98059, the G[alpha]i/G[alpha]q inhibitor peruses toxin (PTX), the Src inhibitor PP2, the EGFR inhibitor AG-1478, the cAMP-dependent protein kinase
(PKA) inhibitor H89, and the G[alpha]s inhibitor NF449 before 12-hr exposure to 10(-9) M BPA, a concentration that provided maximum cell proliferation and then evaluated cell viability using the MTT assay.
In this study we established cooperative interaction between substrate binding sites of the cAMP-dependent protein kinase
catalytic subunit (protein kinase A, EC 2.
The CAMP-dependent protein kinase
substrate Rapt in platelets from patients with obsessive compulsive disorder or schizophrenia.
The enzyme was expressed in Escherichia coli  and thereafter stoichiometrically phosphorylated by the catalytic subunit of cAMP-dependent protein kinase
Initially we studied the participation of the cAMP-dependent protein kinase
9:50 BREAK 10:10 "Phosphorylation of the type I regulatory subunit of cAMP-dependent protein kinase
in rat tissues.
For example, catecholamine rapidly activates the [beta]-adrenergic receptor and the cAMP-dependent protein kinase
A (PKA) axis (McKnight et al.
But the trio acted synergistically to upregulate a regulatory subunit of cAMP-dependent protein kinase
, a ubiquitous protein involved in neurologic pathways and a key regulator of neuronal growth in the clam embryo model.