cDNA


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Related to cDNA: CDNA microarray

cDNA

(cell and molecular biology)
References in periodicals archive ?
The sequence of Wild Argali ISG15 cDNA and the inferred amino acids (Figure 4) were submitted to GenBank as the accession no.
The products of double-stranded cDNA synthesis (1500 ng) were analyzed with 2D-SDE on 6% polyacrylamide gels with 7 mol/L urea (14).
The cDNA generated from the RNA is used in standard PCR with Taq polymerase and gene-specific primers for claudin-2 and claudin-12.
To evaluate the subtraction efficiency, we used subtracted and unsubtracted cDNA pools as templates to amplify GAPDH (about 500 bp).
cDNA display experiments showed the presence of display bands probed that the RNA was functional.
Isolation of the pPICZ[alpha]A/synthetic huIFN[alpha]2 recombinant plasmid containing the cDNA sequence encoding synthetic human IFN[alpha]2a was performed by colony PCR using the 5' FfgsI forward and 3' RIFN[alpha]2 reverse primers (Table 1) as described in reference [17] and by restriction analysis using EcoRI and NotI as recommended by the manufacturer (Amersham Biosciences, Athens, Greece).
For cDNA cloning, the RT-PCR amplified products of pooled whole animal and hemolymph samples of Population #2 and hemolymph from TSV-challenged survivors of cross #17 were run in a 1% agarose gel and eluted using Spin-X column (Costar, MA).
Since 2003, Sarath and collaborator Paul Twigg of the University of Nebraska-Kearney have produced several cDNA libraries from switchgrass.
During the next several weeks blood clotting factors will be generated as P32 labeled or biotin labeled (non-radioactive) cDNA probes to probe Northern RNA blots containing total RNA extracted from adult male rat skeletal muscle exposed to the atrophy of simulated microgravity.
The three-year deal with Japan's Kazusa DNA Research Institute, a leading supplier of high quality cDNA, provides MDS Proteomics with access to one of the richest cDNA collections in the world.
The CDNA celebrates its 30th anniversary this year and is staging four national conferences in order to meet community nurses and health care workers.