Disodium edetate (Reidel Dehan AG sleeze Hannover) sodium chloride NaCl (Merck) sodium hydroxide (Fluka AG) chloroform (Merck) DTNB of Sigma company cadmium acetate (Sigma) Reduced form of GSH of Fluka HCl 35% (Kolchlight) distilled water double refined potassium dihydrogen phosphate (Merck) water for injection (Elexor laboratories).
Table 1: Effect of different concentrations of cadmium acetate on the chemical status of plasma-glutathione with time.
Table 2: Effect of different concentration of cadmium acetate on the chemical status of cytosolic
Table 3: T-test Paried two sample for means in case of effect of cadmium acetate on plasma GHS.
Table 4: T-test Paired two sample for means in case of effect of cadmium acetate on cytosolic fraction GSH.
Cytosolic fraction was exposed to several concentrations of CdAc to investigate the effect of cadmium acetate on cytosolic fraction GSH level.
Statistical analysis applied to the data shows that cadmium acetate has caused depletion in plasma-GSH as well as cytosolic fraction GSH concentration significantly (pless than 0.
22 Exposure of plasma and cytosolic fraction of human blood to various concentrations of cadmium acetate results in marked depression in GSH level of plasma and cytosolic fraction.
A comparison between the effect of cadmium acetate on plasma GSH and cytosolic fraction GSH is very clear in figure 4 and figure 5 respectively which shows that cytosolic fraction GSH has depleted more than depletion in plasma GSH by cadmium acetate indicating the penetrating of Cd2+ in RBC(s) as various concentrations of Cd2+ were incubated with whole blood and later on both the fractions (blood plasma and cytosolic fraction) were separated.