Based on the previous classification, Table III shows the percentage of intact sperm cells that underwent
capacitation along with acrosomal reaction in fresh samples and after incubation in presence of PVL.
This channel is responsible for the alkalization of the intracellular environment and is necessary for
capacitation, acrosome reaction, hyperactivation and sperm motility.
Positive role of reactive oxygen species in mammalian sperm
capacitation: triggering and modulation of phosphorylation events.
They are performing a gut rhetoric--the
capacitation of rhetoric--as a skilled activity of experimenting with probiotics.
Capacitation status was evaluated by dual staining according to a previously described method [10,13,15].
Adiponectin and its receptors are expressed during pre- and postcapacitation of spermatozoa, suggesting that adiponectin might have a role in sperm
capacitation [55].
After a team discussion, the
capacitation of these professionals was the chosen intervention to improve the quality of the data of the SIAB and the performance of these actors in the promotion of breastfeeding.
See "Histological examination of the testes," "Sperm count, motility parameters, and viability," "Assessment of sperm
capacitation status," "Quantitative detection of the intracellular ATP, ROS, Ca2+, and LDH levels," and "Fertility assessment of F1 males" in the Supplemental Material for the detailed procedures of histological examination of the testes, sperm function tests, and fertility assessment of the F1 male mice.
Determination of
Capacitation. An aliquot of 150 [micro]L of BWW medium was placed in a prewarmed culture dish (35 x 10 mm, Corning, New York, USA) and covered with a prewarmed 22 x 22 mm coverslip.
The reactive oxygen species produced by sperm play a key role in many physiological processes such as hyperactivation [24],
capacitation [25], and the interaction between the sperm and oocyte [26].
Moreover, they play the necessary role in sperm
capacitation, acrosome reaction, and fertilization [18-21].
