Here we report a quantitative PCR method, followed by separation by capillary gel electrophoresis
of the fluorescently labeled amplified exons of hot spot regions of the dystrophin gene, which allowed us to detect ~99% patients (affected males and female carriers) with macrodeletions and 89% with macroduplications, and to identify small insertions or deletions in those regions.
High-resolution capillary gel electrophoresis
of reducing oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonate.
This method can easily be implemented into a minimally equipped laboratory, and the detection of the PCR products can be automated by the use of capillary gel electrophoresis
. However, a technical failure in the PCR could lead to misclassification of a patient's Apo E genotype.
Prediction of migration behaviour of oligonucleotides in capillary gel electrophoresis
. J Chromatogr 1992; 593:297-303.
Capillary gel electrophoresis
(CGE) in molecular pathology and genetics is an ideal instrument for fast, fully automated analysis.