Enhancement of cholinergic activity by [M.sub.1] and [M.sub.2] muscarinic receptor agonists carbachol
(22) and pilocarpine (23) or the acetylcholinesterase inhibitor soman (24) produce convulsions in animals.
The [I.sub.K1] blocker Ba[Cl.sub.2], [I.sub.SK] blocker apamin, [I.sub.SK4] blocker TRAM 34, [I.sub.Cl- Ca] blocker niflumic acid, and TRPV1 blocker capsaicipine as well as [K.sub.ACh] activator carbachol
(CCh) did not significantly change APs (Figures 1 and 5).
For assessing the proliferation of coculture system, a 1: 1 combination of INS-1 and NG108-15 cells was seeded in 96-well plates at a density of 5 x [10.sup.3] cells per well, allowed to adhere overnight, and subsequently exposed to different coculture media (100 [micro]l) with or without atropine sulfate (Meilun, Dalian, China) or carbachol
We found similar reduced endothelial-dependent relaxation to cumulative doses of carbachol
in both SHR and SHR MOI aortas compared to WKY aortas, suggesting that MOI was not able to ameliorate endothelial function in conductance arteries (Figure 6(a)).
In experiments, potassium chloride (KCl), histamine dihydrochloride, carbamylcholine chloride (carbachol
), hydroxyzine dihydrochloride, verapamil hydrochloride, and 2-pyridylethylamine were manually diluted in distilled water and added to the organ bath.
Takai, "Two types of non-selective cation channel opened by muscarinic stimulation with carbachol
in bovine ciliary muscle cells," The Journal of Physiology, vol.
Paracentesis was performed through a side port opened with a 15[degrees] blade and 0.10% carbachol
(Miostat, Alcon Laboratories, USA) was administered in the anterior chamber.
Modes of stimulation included the use of carbachol
, isoprenaline and electrical field stimulation (EFS).
The two miotics are acetylcholine (Miochol E) and carbachol
As compared to the control cells, the patient-specific iPSC-derived smooth muscle cells were found to have abnormal organization of alpha-actin filaments, high rate of proliferation and migration, low sensitivity to vasoactive drugs (carbachol
and endothelin-1), and decreased capacity of vessel-like structure formation [4, 69].
In isolated rabbit urinary bladder preparations, Artemisia vulgaris (Av.Cr) crude extract inhibited the contractions produced by carbachol
(CCh, 1 M) and high K+ (80 mM) in a concentration-dependent (0.03-10 mg/mL) manners.
Subjecting the rats to RS, NMS, or NMS/RS resulted in reduced responsiveness of the stomach fundus to the stimulants and relaxants investigated, including carbachol
, potassium chloride, serotonin and adrenaline.