This water-based, solvent-free carbodiimide
crosslinker improves the coating's physical properties, reduces tackiness during stacking and increases cut-through resistance during embossing.
Moreover, processed with calcium chloride solution (for alginate) and mixed solution of l-ethyl-3-(3-dimethyl aminopropyl) carbodiimide
(EDC) and N-Hydroxysuccinimide (NHS) (for gelatin), the composite are able to achieve adequate mechanical property for desired 3D structures .
Collagen was extracted from rat tail tendon, electrospun into fibers, and cross-linked using l-ethyl-3-(3-dimethylaminopropyl) carbodiimide
hydrochloride (EDC) in acetone.
Carboxyl groups on the surface of specifically classified spectral polystyrene microspheres (BioPlex microbeads; Bio-Rad, Hercules, CA, USA) were converted to reactive esters by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
method (Calbiochem, Woburn, MA, USA).
CarboMAX improves standard carbodiimide
chemistry by providing faster activation, less epimerization, and increased stabilization of acid-sensitive linkages (ex.
Hemin, 1-pyrenebutanoic acid, succinimidyl ester (PBSE) was obtained from Setareh Biotech LLC; 1-ethyl-3-(3dimethylaminopropyl) carbodiimide
hydrochloride (EDC) was obtained from TCI America; and N-hydroxysuccinimide [+ or -] 98% (NHS) was obtained from Alfa Aaesar.
Zero-length cross-linking reagent EDC (1-ethyl-3[3-dimethylaminopropyl] carbodiimide
hydrochloride) was used at decreasing concentrations.
Tetraethyl orthosilicate (TEOS, 98%), p-toluenesulfonyl chloride (TsCl), ADA, doxorubicin (DOX, 99%), mPEG (Mw 5000), DTT, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
(EDC, 97%), N,N-dimethyl formamide (DMF), and ethylenediamine (EDA, 99%) were purchased from Sigma-Aldrich (St.
(EDC/EDAC), tris-carboxyethyl phosphine hydrochloride (TCEP), and dithio-DL-threitol (DTT) have also been used for the synthesis of thiolated NPs .
Suh, "Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
cross-linking," Biomaterials, vol.
Amouse monoclonal antibody (Clone ID: MV12/1/C2-2/1), specific to JEV was originally provided by Venture Technology, Penang, Malaysia [20, 21], and treated with ethyl(dimethylaminopropyl) carbodiimide
(EDC) and N-hydroxysuccinimide (NHS) for activation.