The degree of cardiolipin
peroxidation induced in M0LT3 was significantly higher than MCF7 (p < 0.
2]-GPI, had a high affinity for anionic phospholipids, a requirement for binding IgA aCL to cardiolipins
in autoimmune disease.
At increasing concentrations propofol will also prevent the oxygen free radical mediated peroxidation of cardiolipin
Enzyme-linked immunosorbent assays for other APA specificities (including IgA B2GPI antibody, IgA cardiolipin
antibody, prothrombin antibodies, phosphatidylserine antibodies, and phosphatidylcholine antibodies) are also available but are not currently recommended due to uncertain clinical significance (1) (refer to Figure 1 for a laboratory algorithm outlining APS testing).
Mitochondrial mass determination: The preferential binding of NAO to the cardiolipin
moiety in mitochondria was exploited to estimate mitochondrial mass (23,25).
The good news for researchers is that High Performance Thin Layer Chromatography separation and quantification of sphingomylin, PC, PI, PS, PG, PE, cardiolipin
, PA, lyso PC, PI, PS, PG and PE can be achieved.
Phosphatidic acid (PA) and Cardiolipin
(CAR) in the phospholipids fraction were not identified in the [sup.
antibodies were nonsignificant and CRP was 5.
Blood serologic tests indicated an erythrocyte sedimentation rate (ESR) of 62 mm/h and positive antinuclear antibodies (ANA) at a titer of 640 (homogeneous) but negative cryoglobulins, lupus anticoagulant, antineutrophil cytoplasmic antibodies (c-ANCA), and cardiolipin
For patients suspected of having APS, antibodies to both cardiolipin
and (beta)-2-GPI are used to diagnose the condition.
In its natural form, cardiolipin
occurs as a negatively charged lipid found in cardiac tissue.
Results: Pretreatment of MC3T3-E1 cells with paeoniflorin prevented the MG-induced cell death and formation of intracellular reactive oxygen species, cardiolipin
peroxidation, and protein adduct in osteoblastic MC3T3-E1 cells.