Cell-associated virus yield was quantified by real time PCR assay.
The cell-associated virus proliferation in MDCK cells was also suppressed in multiple-cycle growth conditions (Fig.
The level of cell-associated virus was quantified at 3, 6, 9 and 12 h after infection using real time PCR techniques.
Using heteroduplex mobility and tracking assays, scientists concluded that 7 of these 9 patients had viral rebounds that differed genetically from the cell-associated virus
found in their viral reservoir.
The researchers noted that blood samples from LTNPs had less CD4 T cell-associated virus
when compared to control samples from slow progressors.