After incubation, the tubes were centrifuged at 3,000 x g for 10 min at room temperature and the supernatant was pooled into a sterile Tarson 50 ml centrifuge tube
The average mass of the centrifuge tube
+ retained orange oil was 965.
The bioreactor matches the size of a common 50 ml centrifuge tube
The CultiFlask 50 is the same size as a regular 50 mL centrifuge tube
Fifteen gram of the homogenized sample was placed into a 50 mL polyethylene centrifuge tube
Each slurry sample (1 mL) was transferred to a plastic centrifuge tube
(14 mL; Sarstedt, Inc.
The diluted blood was gently layered on 15 mL of Ficoll-Pague in a 20 x 150 mm centrifuge tube
and centrifuged at 3608 for 50 min at room temperature; the supernatant was carefully aspirated and discarded.
The original centrifuge tube
was refilled to 40 ml with formalin solution and both tubes were processed for each host.
Each clot was transferred to a 50-mL, polypropylene, conical centrifuge tube
and stored at -80 [degrees] C until use.
Finally, 10 g of milled sample was transferred to a 50 ml polypropylene centrifuge tube
and remained samples for subsequent tests were placed in plastic containers, labelled and stored at -18[degrees]C .
Inoculated insects were held in a sterile 50-mL centrifuge tube
(USA Scientific, Ocala, FL) containing a single mature sour orange leaf (Citrus aurantium L.
The supernatant (600 [micro]l) was transferred to a clean 2 ml centrifuge tube
, and 200 [micro]l Solution C3 [MO BIO] was added to each sample.