Extraction of DNA template: First, 100 ul blood was poured into 1.5 ml centrifuge tube
and then added with 50 ul concentrated solution.
Then, the supernatant containing the skeletal muscle satellite cells was collected to 15 mL polypropylene centrifuge tube
and centrifuged for 10 min at 500 g, and the cell pellet was resuspended with 10 mL PBS+, centrifuged for 10 min at 500 g to collect the skeletal muscle satellite cells pellet.
Designed for easy one-handed operation the secure leak proof flip cap lid on each centrifuge tube
can also be unscrewed.
Lastly, 70 [micro]L of the supernatant were transferred to a sterile 1.5-mL centrifuge tube
in preparation for qRT-PCR.
The bioreactor matches the size of a common 50 ml centrifuge tube
. Each bioreactor comes with a screw cap with five holes to allow for optimised gas transfer.
The CultiFlask 50 is the same size as a regular 50 mL centrifuge tube
. Each bioreactor comes with a screw cap with five holes to allow for optimized gas transfer; the caps contain a PTFE membrane to serve as a sterile barrier and to minimize liquid loss by evaporation.
Each slurry sample (1 mL) was transferred to a plastic centrifuge tube
(14 mL; Sarstedt, Inc., Numbrecht, Germany) and frozen (-20[degrees]C) until cell lysis and extraction and precipitation of DNA with ethanol was implemented.
7 -- Dynalab has launched a centrifuge tube
rack and box with cover, which protects samples from dust and frost.
A 200-[micro]L aliquot of saline was then removed from the cryotube and transferred to a sterile 1.5-mL centrifuge tube
for DNA extraction.