This assay was similar as mentioned above using a microplate format, but in the absence of
cephalin, which was prepared as follow: 40 [micro]L 0.02 M Tris-HCl / PEG buffer (pH 7.4) + 10 [micro]L SPs (Cc-SP2: 0, 4.1, 41.6 or 83.3 [micro]g well-[plate.sup.-1]; UHEP: 2 [micro]g well-[plate.sup.-1]) + 60 [micro]L 20 mM CaCh / 0.33 mM chromogenic substrate S2238 (10:50 ratio, v/v-1).
Moreover, in his hypercoagulable phase, as evidenced by the kaolin
cephalin clotting time (P<0.05) consumed most full functionally active platelets.
ACT clotting times for plasma samples that had
cephalin (25u1) added back were returned to near normal ACT clotting times, (approx.
(2016) revealed that a native SPs fraction and its various chemically (HCl treated)-modified products from the red seaweed Acanthophora muscoides attenuated coagulation status activated by the use of
cephalin in an in vitro TG continuous system.
During the acute phase, her platelet count fell to 63,100/[mm.sup.3], kaolin
cephalin clotting time (KCCT) was 88 seconds compared to a pooled control KCCT value of 30 seconds, and the international normalized ratio (INR) was 3.
In a series of reports published over the last decade, these authors reported on how to record thrombin generation in vitro after activation of defibrinated plasma by means of intrinsic (
cephalin) or extrinsic (tissue factor) activators.
The kaolin
cephalin time was 43 seconds (control 34 seconds).
TG assay was performed in a microplate format, containing: 10 [micro]L of
cephalin (contact-activator system) + 30 [micro]L 0.02 M Tris HCl/PEG-buffer (pH 7.4) + 10 [micro]L SPs (Cc-SP1: 0, 4.1, 8.3, 41.6 or 83.3 [micro]g well-[plate.sup.-1] or UHEP: 2 [micro]g well-[plate.sup.-1]) + 60 [micro]L 20 mm Ca[Cl.sub.2]/0.33 mm chromogenic substrate S2238 (10:50 ratio, v [v.sup.-1]).
The Quick time was within reference values at 100%,
cephalin kaolin time (CKT), 34 s.
TG assay was performed in a microplate format, containing: 10 [micro]L of
cephalin (contact-activator system) + 30 [micro]L of 0.02 M Tris HCl/PEG-buffer (pH 7.4) + 10 [micro]L of SPs (Am-2 [native]: 0, 4.1, 8.3, 41.6 or 83.3 [micro]g well-plate-1 [hydrolysates-1 in 10 mL of solution], or UHEP: 2 jig well-plate-1) + 60 [micro]L of 20 mM Ca[Cl.sub.2] 0.33-1 mM chromogenic substrate S2238 (10:50 ratio, v v-1).
The partial thromboplastin time (
cephalin time) in anticoagulation therapy.