Our work with soy flour has shown that dry and wet adhesive bond strengths for samples made and tested using this ABES method were remarkably insensitive to the type of soy flour used (Frihart and Satori 2012) or to the addition of chaotropic
agents, salts, surfactants, and co-solvents to the soy flour (Frihart and Lorenz 2013).
Since then, many improvements have been made in the basic silica-based nucleic acid binding and elution chemistry using chaotropic
The filter paper matrix of the FTA Gene Guard system is impregnated with a chaotropic
agent that denatures infectious agents, and thus samples are no longer considered a biohazard.
On the other hand, the addition of chaotropic
salts leads to diminished hydrogen bonding, which can be accompanied by decreased hydrophobic associations.
The patented TruTip family of products uses a porous nucleic acid binding matrix embedded in a pipette tip and extracts DNA or Ribonucleic acid (RNA) using well-understood chaotropic
The list of substances which have been shown to inhibit DNA polymerases is long, but some of the most commonly encountered problem substances in clinical specimens include heme (and thus whole blood); chelating agents (including many anticoagulants, whose mode of action is by Ca++/Mg++ chelation; removal of these from the polymerase buffer makes the dNTPs less reactive); ethanol or isopropanol; and protein denaturing or chaotropic
agents such as guanidine or urea.
agents (urea, guanidine hydrochloride, and dicyandiamide), surfactants (sodium dodecyl sulfate or cetyltrimethylammonium bromide), and the cosolvent propylene glycol were all expected to provide increased protein-protein and protein-PAE interactions.
Given these multiple steps, conventional methods are relatively laborious compared with methods of DNA extraction on a solid substrate, in which DNA binds to a silica surface in solutions containing chaotropic
salts, such as those of iodide or perchlorate (16).
Each pool was ground in RNA NOW chaotropic
solution (Ozyme, Montigny le Bretonneux, France).
Effects and agents driving and facilitating the thiol/disulfide exchange reaction are higher temperature, denaturating agents like SDS, chaotropic
ions, and basic conditions.
In addition, since Versagene reagents contain no chaotropic
reagents, waste can be discarded without hazardous materials requirements.
The patented TruTip family of products uses a porous nucleic acid binding matrix embedded in a pipette tip, and extracts DNA or ribonucleic acid (RNA) using well-understood chaotropic