The rate of the conversion of CPRG to chlorophenol red (CPR) was measured bichromatically at 577 and 700 nm.
 Nonstandard abbreviations: KLH, keyhole limpet hemocyanin; ACMIA, affinity column mediated immunoassay; WB, whole blood; CPRG, chlorophenol red-[beta]-D-galactopyranoside; CPR, chlorophenol red; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LOB, limit of blank; LOD, limit of detection; LOQ, limit of quantification; MVIII, metabolite VIII.
Mucoadhesion retention time of PSY and TSPY gels was comparatively evaluated by applying 1g of the gels containing a dye, chlorophenol red
(0.01%, w/v) on a chicken buccal tissue glued to a glass slide.
Estrogenic activity from the enzymatic hydrolysis of chlorophenol red
[beta]-D-galactopyranoside was read at 540 nm using a colorimetric assay.
This [beta]-galactosidase activity is quantified at 540 nm by the conversion of the chromogenic substance chlorophenol red-[beta]-D-galactopyranoside into chlorophenol red
. The bioassay response is expressed as the absorbance at 540 nm divided by the optical density at 630 nm [[(A540/A630).sub.net]].
Other features include auto shut-off after 10 minutes of non-use, self-diagnostic and error messages, chlorophenol red
method and is a technological alternative to chemical test kits.
[beta]-D-galactopyranoside (CPRG) and p-nitrophenylphosphate (PNPP) were purchased from Roche (Mannheim, Germany).
[beta]-D-galactopyranoside (CPRG) was obtained from Roche Diagnostics (Mannheim, Germany).
The supernatant containing the CsA-antibody-enzyme complex is then transferred to a measuring cuvette, where it is mixed with chlorophenol red
galactoside, which is hydrolyzed by the [beta]-galactosidase to chlorophenol red
CPRG is converted to chlorophenol red and reflects [Beta]-galactosidase activity, which is indicative of the estrogenic activity.
conversion of the chromogenic substance chlorophenol red-[Beta]-D-galactopyranoside (CPRG) into chlorophenol red by the action of the reporter enzyme [Beta]-galactosidase.
[Beta]-Dgalactopyranoside (CPRG) was obtained from Boehringer (Mannheim, Germany).