Quantification of CrAQP mRNA through qPCR indicated that the transcript level was higher in the ectosome than in the choanosome.
reniformis as it relates to the engulfed siliceous particles; taking a molecular approach, we identified and cloned the full-length cDNA that encodes the sponge aquaporin CrAQP, whose transcript level was assayed in the ectosome and choanosome of C.
The amount of CrAqp mRNA in the two main regions of Chondrosia reniformis, the ectosome and choanosome, was analyzed using a qPCR approach with Chromo 4 Real-Time PCR Detector (MJ Research PTC200; Labcare Service, Braintree, UK).
This species was originally described as having the choanosome
light purple and the ectosome white semi-transparent.
In contrast, the choanosome, or inner sponge layer, is virtually devoid of zooxanthellae.
In the laboratory, the zooxanthellaedense pinacoderm (Rutzler, 1990) was separated from each sample, leaving the choanosome, which is devoid of zooxanthellae.
For zooxanthellae samples, the outer pinacoderm layer containing the zooxanthellae was physically separated from the inner choanosome by using straight-edge razor blades.
Anecdotal evidence indicates that the cortex of forma rigida and forma incrustans protects the choanosome
In fact, most sponges from Terra Nova Bay (Ross Sea, Antarctica) host large populations of both planktonic and benthic diatoms, and these microorganisms are actively taken up by the pinacoderm and thus enter the sponge choanosome
A section through the sponge reveals two distinct regions: a cortical zone called ectosome, and an internal zone, the choanosome, which contains the choanocyte chambers.
To verify that the polarization between the upper and lower ectosome arises very early in development, experiments were performed with half-cores; these are fractions of sponge tissue that contain a portion of choanosome covered on one side by either upper or lower ectosome [ILLUSTRATION FOR FIGURE 1 OMITTED].
Ultrastructure of choanosome
and sponge classification.