These cells, known as chondrocytes
, are returned into the patient's defect area in a second surgical procedure.
In vitro effects of platelet-rich gel supernatants on histology and chondrocyte
apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide.
MIA, a derivative of acetic acid, causes chondrocyte
apoptosis by inhibiting glyceraldehyde-3-phosphate dehydrogenase and is considered the most appropriate method for inducing OA changes4.
Reconstruction of alar nasal cartilage defects using a tissue engineering technique based on a combined use of autologous chondrocyte
micro-grafts and platelet-rich plasma.
We did the culture in a 6 well's plate to observe the ability of the MSC to differentiate into a chondrocyte
. To know the ability of the chondrocyte
maintained it's viability capacity in a small container; we did the observation in a 96-wells-plate.
Using these agarose constructs, we confirmed that mechanical stress, both independent and/or in combination with verapamil, can increase chondrocyte
and cartilage replacement represent potential new therapies for treatment of OA and a broad range of degenerative and debilitating diseases that could dramatically reduce the need for mechanical devices.
These changes included disarranged chondrocytes
with degeneration or necrosis, ulcerations in the deep cartilage layer, and fibroplasia at the bottom of the ulcers (Figure 1a).
Subsequently, the constructs can be pre-cultured in vitro to stimulate chondrocyte
differentiation before implantation.
P Sudkamp, and P Niemeyer, "Autologous chondrocyte
implantation in children and adolescents," Knee Surgery, Sports Traumatology, Arthroscopy, vol.
The defect of the control group remained thin, presenting no chondrocyte
proliferation around the perforation (Figure 3(a)).
Schmal, "In-vitro chondrogenic potential of synovial stem cells and chondrocytes
allocated for autologous chondrocyte
implantation--a comparison: synovial stem cells as an alternative cell source for autologous chondrocyte
implantation," International Orthopaedics, vol.