chromomycin

chromomycin

[¦krō·mō′mī·sən]
(microbiology)
Any of five components of an antibiotic complex produced by a strain of Streptomyces griseus; components are designated A1 to A5, of which A3(C51H72O32) is biologically active.
References in periodicals archive ?
Normal sperm morphology and chromatin packaging: comparison between aniline blue and chromomycin A3 staining.
DAPI, DIPI, Hoechst 33258, and Hoechst 33342 bind to AT-rich regions whereas chromomycin A mithramycin, and olivomycin bind to GC-rich regions.
Base-specifc fuorochrome staining using Chromomycin A3 (CMA3) and 4'6-diamidino-2-phenylindole (DAPI) was used to detect GC- and AT-rich regions, respectively, while Distamycin A (DA) was added as counter stainer (Schweizer 1980).
AT-specific 4', 6-diamidino-2-phenylindole (DAPI) and GC-specific chromomycin [A.sub.3] ([CMA.sub.3]) fluorochromes were used for differential staining to reveal AT-enriched heterochromatin patterns and GC-enriched heterochromatic clusters in the nuclear organizer regions (NORs) on chromosomes 1 and 6 in the Ae.
Alternatively, after staining with DAPI, further staining was performed with chromomycin A3 (CMA) at a concentration of 0.5 [micro]g/mL, followed by mounting in glycerol.
In human, percentage of sperm stained positively with Chromomycin A3, an indication of deprotamination, had a positively correlation with macrocephaly sperm [14].
Fluorescent chromosome banding to reveal the type, amount, size and distribution of Het was performed according to the triple staining technique (CDD) of Schweizer and Ambros (1994), using the fluorochromes chromomycin A3 (CMA), distamycin A (DA) and 4-6-diamidino-2-phenylindole (DAPI).
Pirarubicin ([IC.sub.50] = 0.6 [micro]M) and valrubicin ([IC.sub.50] = 1.9 [micro]M) were the most potent compounds in this group; aclarubicin hydrochloride, plicamycin, and the structurally related chromomycin A3 were also active, whereas adriamycin hydrochloride was inactive.
Rutigliano et al., "The DNA-binding drugs mithramycin and chromomycin are powerful inducers of erythroid differentiation of human K562 cells," British Journal of Haematology, vol.
In addition to the conventional staining, the chromosome preparations were also stained with chromomycin ([CMA.sub.3]) and 4',6-diamidino-2-phenylindole (DAPI) fluorochromes [26] in order to identify regions rich in GC- or AT-, respectively.
Several tests such as TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay, aniline blue straining, chromomycin A3 and acridine orange (AO) staining have been described to determine the extent of DNA damage.