Alternatively, after staining with DAPI, further staining was performed with chromomycin
A3 (CMA) at a concentration of 0.
In human, percentage of sperm stained positively with Chromomycin
A3, an indication of deprotamination, had a positively correlation with macrocephaly sperm .
Fluorescent chromosome banding to reveal the type, amount, size and distribution of Het was performed according to the triple staining technique (CDD) of Schweizer and Ambros (1994), using the fluorochromes chromomycin
A3 (CMA), distamycin A (DA) and 4-6-diamidino-2-phenylindole (DAPI).
9 [micro]M) were the most potent compounds in this group; aclarubicin hydrochloride, plicamycin, and the structurally related chromomycin
A3 were also active, whereas adriamycin hydrochloride was inactive.
The GC and AT rich regions were detected using the fluorochromes Chromomycin
The DNA-binding drugs mithramycin and chromomycin
are powerful inducers of erythroid differentiation of human K562 cells," British Journal of Haematology, vol.
Several tests such as TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay, aniline blue straining, chromomycin
A3 and acridine orange (AO) staining have been described to determine the extent of DNA damage.
Notorious examples include 4'-6-diamidino-2-phenylindole (DAPI), which highlights AT-rich regions, and chromomycin
A3 (CMA3) is a guanine-cytosine-specific fluorochrome and indicative of poor chromatin packaged in human sperm via indirect counting of protamine-deficient sperm.
Additionally, for detection of the AT- and GC- rich chromatin regions, some chromosomal preparations were stained with the fluorochromes 4'-6-diamidino-2-phenylindole (DAPI) and chromomycin
Staining with aniline blue, toludine blue, and chromomycin
A3 is used to identify sperm chromatin packaging defects.
Differential fluorescent staining of porcine heterochromatin by chromomycin
A3/distamycine A/DAPI and D287/170.