Interestingly, CENP-E is involved in the initial alignment of chromosomes at the spindle equator on the metaphase plate, and therefore, it is required for steady spindle microtubule capture at the kinetochores, which is a critical step in proper chromosome congression during prometaphase.
Basically, siRNA-mediated CENP-E silencing results in the failure of chromosome congression to the equator, which is characterized by the clustering of chromosomes near the poles (5,6).