The granted patent issued by the USPTO now confers exclusive rights to the Company's lead product, PRP, by demonstrating that compositions comprising trypsinogen and chymotrypsinogen
exhibit a synergistic ability to inhibit the growth of various cancer cell lines.
The remarkable achievement to identify and develop a suitable method to measure four key analytes from PRP, the two proenzymes, trypsinogen and chymotrypsinogen
, as well as the activated enzymes, trypsin and chymotrypsin, is particularly significant due to the close structural similarity of each protein structure.
The column had been calibrated with molecular mass markers, including blue dextran 2000 (to determine void volume), bovine serum albumin (67 kDa), ovalbumin (43 kDa), chymotrypsinogen
A (25 kDa), myoglobulin (17.6 kDa), ribonuclease A (13.7 kDa), aprotinin (6.5 kDa) and vitamin B12 (1.3 kDa) (GE Healthcare).
IDDM patients' sera recognize a novel 30-kD pancreatic autoantigen related to chymotrypsinogen
. Immunol Invest 1993; 22:219-27.
(4) (p227) Trypsin can then rapidly begin activating other trypsinogen and chymotrypsinogen
molecules in a cascade effect.
Trypsinogen and chymotrypsinogen
in the homogenates were activated with enterokinase at a concentration of 4 mg/ml and incubated at 4[degrees]C for 24 h.
Johnson A, Hurwitz R, Kretchmer N (1977) Adaptation of rat pancreatic amylase and chymotrypsinogen
to changes in diet.
According to the company, the scientific presentations establishes that compositions comprising trypsinogen and chymotrypsinogen
exhibit a synergistic ability to inhibit the growth of various cancer cell lines and that this effect would be unexpected to one of ordinary skill in the art, thus concluding the claims were patent-able.
The column was calibrated with ovalbumin (43 kDa), chymotrypsinogen
A (25 kDa), and ribonuclease A (14 kDa) (Amersham Biosciences).
Requirements for activation of trypsinogen and chymotrypsinogen
in rabbit pancreatic juice.