Simmon's citrate test: Simmon's citrate slants in test tubes were stab inoculated in same way as SIM media and incubated at 37[degrees]C for a week after which the findings were recorded [22,23].
The finding of one isolate negative for indole production and positive in Simmons citrate test, however, indicates atypical strain which was also reported in previous studies where variation in Simmons citrate utilization [7,32,33] and indole production  was reported among E.
BSH lb (Lactobacillus sp.) characterized on the basis of biochemical tests whose antibacterial activity zone was of 2 mm found to be negative for catalase test (no bubble formation), negative for indole test, and negative for nitrate reduction test, citrate test, methyl red Voges-Proskeur test, casein hydrolysis test and for starch agar test (Table 1).
It also showed negative result for catalase test, nitrate reduction test, citrate test, methyl red Voges-Proskeur test, casein hydrolysis test but positive result for glucose, lactose and sucrose fermentation (Table 1).
Biochemical test Klebsiella Escherichia pneumonia coli Catalase test (+)ve (+) ve Oxidase test (-)ve (-) ve simmon citrate test
(+)ve (-) ve Urease test (+)ve Yellow/Yellow with gas production(-)ve TSI (+) ve (+) ve Indol test (-)ve (+) ve Motility test (-)ve (+)ve Methyl red test (-)ve (+) ve Vogas-proskaure test (+) ve (-) ve Gram stain (-)ve (-) ve Cell morphology On Macconkey agar Smooth, glassy and bacterial colonies translucent, rosy have a viscous/mucoid pink on MacConkey appearance with the appearance of metallic sheen when growing on Eosin methylene blue.
Tests such as coagulase tests, DNAase tests, Optochin test, bile solubility, bacitracin test, triple sugar iron test, indole test, citrate test
, urease test, oxidase, methyl red(MR) and voges--proskauer (VP) tests were done.
Organisms MR-Test VP-Test Simmon Mannitol Motility (Isolates) citrate Test
Test C1 +ve -ve +ve -ve +ve C2 -ve -ve -ve -ve -ve C3 +ve -ve +ve -ve +ve V1 +ve -ve -ve -ve +ve A1 +ve -ve -ve -ve -ve A2 +ve +ve +ve +ve +ve A3 +ve -ve +ve +ve -ve A4 -ve -ve -ve +ve +ve A5 +ve -ve +ve +ve +ve A6 -ve +ve +ve +ve +ve A7 -ve -ve -ve +ve +ve Table 5: Extracellular Enzyme Activity of Bacterial Isolates.
Indole test, Methyl Red-Voges- Proskaeur test (MR-VP) and Citrate test
were also performed for further confirmation of E.
In addition biochemical tests like Catalase, Oxidase, acid from glucose, Kossar's Citrate test
for citrate utilization were also carried out.
Biochemical test Klebsiella Escherichia pneumonia coli Catalase test + ve + ve Oxidase test - ve - ve simmon citrate test
+ ve - ve Urease test + ve Yellow/Yellow with gas production(-)ve TSI + ve + ve Indol test - ve + ve Motility test - ve + ve Methyl red test - ve + ve Vogas-proskaure test + ve - ve Gram stain - ve - ve Cell morphology On Macconkey agar Smooth, glassy and bacterial colonies translucent, rosy have a viscous/ pink on MacConkey mucoid appearance with the appearance of metallic sheen when growing on Eosin methylene blue.
Gallium citrate tests
take 48-72 hours to complete and "we almost always equivocate [over the results]," and CT scans are "really not that helpful," he said.