Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis
in Wistar rat bone marrow cells.
Hypothesis: EUG promotes a burst in ROS production causing cell-cycle perturbations, mitochondria toxicity and clastogenesis
triggering apoptosis in melanoma breast- and cervix-cancer cells in vitro.
It has several advantages over CA test such as additional detection of aneugens, technically less demanding, ease of scoring and possibility of automation and micronuclei arise from two important types of genetic damage, i.e., clastogenesis
and spindle disruption.
Ascorbate acts as a highly potent inducer of chromate mutagenesis and clastogenesis
: linkage to DNA breaks in G2 phase by mismatch repair.
El test de micronucleos (MN) detecta perdidas cromosomicas debidas a defectos en la segregacion de las cromatidas que llevan a la no disyuncion o perdida cromosomica (aneugenesis) o fragmentos originados por roturas de la cromatina (clastogenesis
Attia, "The impact of quercetin on cisplatin-induced clastogenesis
and apoptosis in murine marrow cells," Mutagenesis, vol.
Metabolic activation systems containing 9000g supernatant fraction (S9) of liver homogenates prepared from polychlorinated biphenyl or phenobarbital plus 5,6-benzoflavonepretreated rats were required for mutagenesis and clastogenesis
. Steviol was weakly positive in the umu test using Salmonella typhimurium TA1535/pSK1002 with or without the metabolic system and was negative in other assays (even in the presence of the S9 activation system) using Salmonella typhimurium TA97, TA98, TA100, TA102, TA104, TA1535, TA1537 and Escherichia coli WP2 uvrA/pKM101 and the rec-assay using Bacillus subtilis and in the mouse micronucleus test .
As a result of genetic damage, i.e., damage to the chromosomes, fragments lagging in the course of anaphase or lagging acentric chromosomes or cytoplasmic chromatin-containing bodies are failed to be incorporated into daughter nuclei (clastogenesis
), results in the development of micronuclei in red blood cells (Sharaf et al.,2010).
In this review, the scope has been confined to actual data obtained from research on the modulatory effects of plant extracts on mutagenesis and clastogenesis
- two genotoxic phenomena associated with carcinogenesis.
Bianchi, "Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis
in Wistar rat bone marrow cells," Mutation Research, vol.
Several potential carcinogenic mechanisms have been proposed, including inhibition of DNA repair enzymes, tumor promoter-like induction of proliferation, alteration of DNA methylation, and clastogenesis