clathrin-coated pit

clathrin-coated pit

[¦klath·rən ‚kōd·əd ′pit]
(cell and molecular biology)
A partially invaginated membrane structure (bud or pit) involved in receptor-mediated endocytosis consisting of a cluster of receptor proteins attached on the cytosolic side by means of adaptin molecules to the protein clathrin, which forms a lattice-shaped coating. Complete invagination of the pit and release from the membrane results in a clathrin-coated vesicle containing cargo molecules.
References in periodicals archive ?
Investigating the Effects of Clathrin-Coated Pit Size Distribution of Productive and Abortive Pit Lifetimes.
This process of clathrin-mediated endocytosis will first result in clathrin-coated pits (CCPs).
Lou et al., "Recruitment of endophilin to clathrin-coated pit necks is required for efficient vesicle uncoating after fission," Neuron, vol.
The team experimented on clathrin-coated pits and narrowed down the location of the protein in the pit and on the inside of the cell's surface.
In Chinese hamster ovary (CHO) cells, the oxidative state of cell surface cholesterol is one of the factors involved in endothelin receptor type A (ETA) internalization, which occurs via caveolae or clathrin-coated pits [25].
Masaki, "Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells," The Journal of Biological Chemistry, vol.
The clathrin adaptor AP-2 is a heterotetrameric complex formed by [alpha], [beta]2, [mu]2, and [sigma]2 adaptin subunits and has critical functions in the assembly and recruitment of cargo proteins to clathrin-coated pits. The [mu]2-adaptin subunit of AP-2 binds directly to tyrosine-based motifs within the cytoplasmic- (C-) tail domain of a number of GPCRs including PAR1 [16] and is required for constitutive internalization and cellular resensitization to thrombin [13].
Since calpains are involved in the formation of coated vesicles and [alpha]-spectrin degradation is essential for the endocytic process, our data indicate that PAR1 signals to promote the internalization of PAR1 carried by clathrin-coated pits [47].
Electron microscopic examination of the cells showed gold particles being endocytosed via clathrin-coated pits in 16HBE cells, indicating the presence of functional mannose receptors (Figure 1(b)) [17].
We present evidence that a functional MR family member is expressed in human bronchial epithelial cells; this is supported by endocytosis of mannosylated neoglycoprotein in clathrin-coated pits. This adds to the list of MR in the epithelium: retinal pigment epithelial cells [23] and nasal polyposis [24].
The mature glycosylated protein ([M.sub.r] 160 000) is transported to the cell surface where it clusters in clathrin-coated pits (4).
Sequence variations that encode receptors that move to the cell surface and bind LDL normally but are unable to cluster in clathrin-coated pits are denoted class 4 sequence variations (6, 12).

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