Samal, "Role of fusion protein cleavage site
in the virulence of Newcastle disease virus," Microbial Pathogenesis, vol.
Vitellogenin from these sea stars contains characteristic Vtg sequences, protein domains, and shared features, such as multiple furin/subtilisin cleavage sites
However, later studies showed that the P1 and/or P1' positions of the major cleavage sites
in a combinatorial peptide mixture were occupied by bulky hydrophobic or aromatic residues such as Leu, Phe, Tyr etc.
All identified ErbB2 peptides were imported into Skyline for each digestion condition and corresponding spectral libraries were made with no filtering for the types of modifications or cleavage sites
of the peptides.
(13) This is remarkable because the mutation is distant from the stalk cleavage site
and probably causes a change in the relative orientation of the two domains, allowing better access of the sheddase.
dubia, since the latter species has two cleavage sites
for the enzyme SalI, no restriction site for the enzyme ApaI and different site position for the enzyme EcoRI (Figure 4).
University of California (Oakland, CA) has patented an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site
of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor.
Maturation of the precursor proteins begins by intramolecular cleavage of Gag-Pro-Pol and continues by a process of ordered cleavages governed by differences in the inherent processing rates at each cleavage site
(see Figure 3) [14-18] which for HIV Gag can vary up to 400-fold [16,17].
No negative effects of the FTA[R] card inactivation on the nucleotide sequences were observed, allowing the determination of its virulence by direct nucleotide sequencing of the F protein cleavage site
, resulting in a lentogenic strain in concordance with the initial virus applied on the cards.
This technology involves removal of several of the amino acids found at the hemagglutinin cleavage site
, growing plasmids that code for the modified components, and then reassorting them into a nonpathogenic strain, Dr.
A mild infiltrate of chronic inflammatory cells, including lymphocytes and plasma cells, is noted subjacent to the cleavage site
. Direct immunofluorescence studies demonstrate a linear band of immunoglobulins (IgG) and complement (C3) along the basement membrane zone in 90% of affected patients.