restriction site

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restriction site

[ri′strik·shən ‚sīt]
(cell and molecular biology)
A sequence in a deoxyribonucleic acid molecule that can be cleaved with a specific restriction endonuclease.
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References in periodicals archive ?
Samal, "Role of fusion protein cleavage site in the virulence of Newcastle disease virus," Microbial Pathogenesis, vol.
Vitellogenin from these sea stars contains characteristic Vtg sequences, protein domains, and shared features, such as multiple furin/subtilisin cleavage sites for processing.
However, later studies showed that the P1 and/or P1' positions of the major cleavage sites in a combinatorial peptide mixture were occupied by bulky hydrophobic or aromatic residues such as Leu, Phe, Tyr etc.
All identified ErbB2 peptides were imported into Skyline for each digestion condition and corresponding spectral libraries were made with no filtering for the types of modifications or cleavage sites of the peptides.
(13) This is remarkable because the mutation is distant from the stalk cleavage site and probably causes a change in the relative orientation of the two domains, allowing better access of the sheddase.
University of California (Oakland, CA) has patented an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor.
Maturation of the precursor proteins begins by intramolecular cleavage of Gag-Pro-Pol and continues by a process of ordered cleavages governed by differences in the inherent processing rates at each cleavage site (see Figure 3) [14-18] which for HIV Gag can vary up to 400-fold [16,17].
No negative effects of the FTA[R] card inactivation on the nucleotide sequences were observed, allowing the determination of its virulence by direct nucleotide sequencing of the F protein cleavage site, resulting in a lentogenic strain in concordance with the initial virus applied on the cards.
This technology involves removal of several of the amino acids found at the hemagglutinin cleavage site, growing plasmids that code for the modified components, and then reassorting them into a nonpathogenic strain, Dr.
A mild infiltrate of chronic inflammatory cells, including lymphocytes and plasma cells, is noted subjacent to the cleavage site. Direct immunofluorescence studies demonstrate a linear band of immunoglobulins (IgG) and complement (C3) along the basement membrane zone in 90% of affected patients.