cloning vector

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Related to cloning vector: expression vector

cloning vector

[′klōn·iŋ ‚vek·tər]

(cell and molecular biology)

A carrier, such as a bacterial plasmid or bacteriophage, used to insert a genetic sequence, such as a deoxyribonucleic acid fragment or a complete gene, into a host cell such that the foreign genetic material is capable of replication.

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References in periodicals archive ?

The purified PCR products were ligated into pMD-18T cloning vector (Takara Biotechnology, China).

Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA

After moving to National Institutes of Health as Visiting Scientist in 1983, he developed a high-efficiency transfection method for delivering cDNA libraries into mammalian cells and cDNA cloning vectors that enable selection on stable cDNA expression.

Functional cDNA expression cloning: pushing it to the limit

The amplified gene of cellulase was purified from the agarose gel after electrophoresis using Qiagen gel extraction kit and ligated into the pTZ57R/T cloning vector (Fermentas) using DNA ligase and ligation buffer.

Cloning of a cellulase gene from indigenous strain of bacillus species

After purification of PCR product from the gel, both fragment and STV28 cloning vector were doubled digested with HindIII and PstI.

Isolation and identification of a native rhodococcus strain producing cholesterol oxidase from soil

A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene.

Carbapenemase-producing Enterobacteriaceae, U.S. rivers

The cloning vector itself is predicted to generate a peptide of only 4.1 kDa consisting of the N-terminal UE sequence only; the fact that this is not regularly observed to accumulate on induction of nonrecombinant control samples may be attributable to its small size or to the fact that this sequence ends in a rather hydrophobic sequence (SLPGG), which may contribute to instability (12).

Detection of cystic fibrosis mutations by peptide mass signature genotyping

The deduced nucleotide sequence of avimer molecule was synthesized and cloned into pGH cloning vector by GENEray Co.

Novel Recombinant Traceable c-Met Antagonist-Avimer Antibody Mimetic Obtained by Bacterial Expression Analysis

The PCR products of three target cDNAs were cloned in [p.sup.DRIVE] cloning vector (Qiagen, Germany) and sequence confirmed for each cDNA.

Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/l, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats

The amplifications were ligated in pTZ57R/T cloning vector. Vector containing products were transformed further into E.

First Report of the Molecular Characterization of the Endosymbiont Candidatus portiera aleyrodidarum from Cotton Whiteflies Collected from Pakistan

This fragment was inserted into the pTG19-T PCR cloning vector (SinaClon, Iran) during T/A cloning procedure using DNA Ligation Kit, Mighty Mix (TaKaRa, Japan) as described in manufacturer manual (Figure 1).

SITE-DIRECTED MUTAGENESIS OF THE BACTERIAL PHYTASE GENE COMPATIBLE FOR CHICKEN CODON PREFERENCES

The amplified genes were cloned in pTZ57R TA cloning vector sequenced and submitted to EMBL with accession nos.

Molecular Cloning and Characterization of Exochitinase A Gene of Indigenous Bacillus thuringiensis Isolates

The purified PCR products were ligated into pMD-18T cloning vector (Takara Biotechnology, Dalian, China), transformed and sub-cultured.