cloning vector


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Related to cloning vector: expression vector

cloning vector

[′klōn·iŋ ‚vek·tər]
(cell and molecular biology)
A carrier, such as a bacterial plasmid or bacteriophage, used to insert a genetic sequence, such as a deoxyribonucleic acid fragment or a complete gene, into a host cell such that the foreign genetic material is capable of replication.
References in periodicals archive ?
The purified PCR products were ligated into pMD-18T cloning vector (Takara Biotechnology, China).
Enzymatic digestion of pUC18 cloning vector and pET-24a expression vector
The amplified gene of cellulase was purified from the agarose gel after electrophoresis using Qiagen gel extraction kit and ligated into the pTZ57R/T cloning vector (Fermentas) using DNA ligase and ligation buffer.
First the PCR amplified product was cloned in the TA cloning vector pTZ57R (data not shown) and subsequently subcloned in the yeast shuttle vector pGAL426.
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
The downstream primers for pSPPO1 (5'-CTC ACA ATT CCA CAC AAC ATA C-3') and for pSPPO2 (5'-GGC TTC GCA TCC CTG TGG-3') were in the fragment of cloning vector between the promoter and the PPO sense gene, and in the sense PPO gene, respectively.
The cloning vector itself is predicted to generate a peptide of only 4.
Then S1 gene was T/A ligated into the cloning vector pMD-18T (TaKaRa, China), keeping at 16oC for 18h in Eppendrof Thermomixer comfort (Eppendrof, New Burnswick,USA).
pGEMT-easy/CD19 recombinant cloning vector was digested by KpnI and HindIII restriction enzymes.
The amplified genes were cloned in pTZ57R TA cloning vector sequenced and submitted to EMBL with accession nos.
These have been cloned into a standard BAC cloning vector.