The purified PCR products were ligated into pMD-18T
cloning vector (Takara Biotechnology, China).
After moving to National Institutes of Health as Visiting Scientist in 1983, he developed a high-efficiency transfection method for delivering cDNA libraries into mammalian cells and cDNA
cloning vectors that enable selection on stable cDNA expression.
The amplified gene of cellulase was purified from the agarose gel after electrophoresis using Qiagen gel extraction kit and ligated into the pTZ57R/T
cloning vector (Fermentas) using DNA ligase and ligation buffer.
After purification of PCR product from the gel, both fragment and STV28
cloning vector were doubled digested with HindIII and PstI.
A pSC101-derived plasmid which shows no sequence homology to other commonly used
cloning vectors. Gene.
The
cloning vector itself is predicted to generate a peptide of only 4.1 kDa consisting of the N-terminal UE sequence only; the fact that this is not regularly observed to accumulate on induction of nonrecombinant control samples may be attributable to its small size or to the fact that this sequence ends in a rather hydrophobic sequence (SLPGG), which may contribute to instability (12).
The deduced nucleotide sequence of avimer molecule was synthesized and cloned into pGH
cloning vector by GENEray Co.
The PCR products of three target cDNAs were cloned in [p.sup.DRIVE]
cloning vector (Qiagen, Germany) and sequence confirmed for each cDNA.
The amplifications were ligated in pTZ57R/T
cloning vector. Vector containing products were transformed further into E.
This fragment was inserted into the pTG19-T PCR
cloning vector (SinaClon, Iran) during T/A cloning procedure using DNA Ligation Kit, Mighty Mix (TaKaRa, Japan) as described in manufacturer manual (Figure 1).
The amplified genes were cloned in pTZ57R TA
cloning vector sequenced and submitted to EMBL with accession nos.
The purified PCR products were ligated into pMD-18T
cloning vector (Takara Biotechnology, Dalian, China), transformed and sub-cultured.
