cloning vector


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Related to cloning vector: expression vector

cloning vector

[′klōn·iŋ ‚vek·tər]
(cell and molecular biology)
A carrier, such as a bacterial plasmid or bacteriophage, used to insert a genetic sequence, such as a deoxyribonucleic acid fragment or a complete gene, into a host cell such that the foreign genetic material is capable of replication.
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The purified PCR products were ligated into pMD-18T cloning vector (Takara Biotechnology, China).
Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA
Enzymatic digestion of pUC18 cloning vector and pET-24a expression vector
Expression of recombinant human insulin-like growth factor type 1 (rhIGF-1) in Escherichia coli
The amplified gene of cellulase was purified from the agarose gel after electrophoresis using Qiagen gel extraction kit and ligated into the pTZ57R/T cloning vector (Fermentas) using DNA ligase and ligation buffer.
Cloning of a cellulase gene from indigenous strain of bacillus species
Purified PCR product was cloned into STV28 cloning vector.
Isolation and identification of a native rhodococcus strain producing cholesterol oxidase from soil
First the PCR amplified product was cloned in the TA cloning vector pTZ57R (data not shown) and subsequently subcloned in the yeast shuttle vector pGAL426.
Production of immunogenic human papillomavirus-16 major capsid protein derived virus like particles
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
Carbapenemase-producing Enterobacteriaceae, U.S. rivers
The downstream primers for pSPPO1 (5'-CTC ACA ATT CCA CAC AAC ATA C-3') and for pSPPO2 (5'-GGC TTC GCA TCC CTG TGG-3') were in the fragment of cloning vector between the promoter and the PPO sense gene, and in the sense PPO gene, respectively.
Overexpression of polyphenol oxidase in transgenic sugarcane results in darker juice and raw sugar
The cloning vector itself is predicted to generate a peptide of only 4.
Detection of cystic fibrosis mutations by peptide mass signature genotyping
Then S1 gene was T/A ligated into the cloning vector pMD-18T (TaKaRa, China), keeping at 16oC for 18h in Eppendrof Thermomixer comfort (Eppendrof, New Burnswick,USA).
CONSTRUCTION AND EXPRESSION OF RECOMBINANT PLASMIDS CONTAINING NEPHROPATHOGENIC IBV S1 GENE AND LI-KEY SEGMENT OF CHICKEN MAJOR HISTOCOMPATIBILITY COMPLEX II GENE
pGEMT-easy/CD19 recombinant cloning vector was digested by KpnI and HindIII restriction enzymes.
Cloning and expression of CD19, a human B-cell marker in NIH-3T3 cell line
The amplified genes were cloned in pTZ57R TA cloning vector sequenced and submitted to EMBL with accession nos.
Molecular Cloning and Characterization of Exochitinase A Gene of Indigenous Bacillus thuringiensis Isolates
These have been cloned into a standard BAC cloning vector.
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