aureus identification, coagulase production (coagulase test
) has been considered as an important criterion for phenotypic identification (Upadhyay et al.
All the isolates were also subjected to tube coagulase test
They included: Indole test, methyl red test (MR), citrate utilization, catalase test, oxidase test, coagulase test
, oxidation--fermentation test, fermentation of sugars, and motility test.
During the identification coagulase test
was carried out.
Gram positive Staphylococci colonies with a positive coagulase test
(17) were inoculated onto Mueller-Hinton agar containing 6 ig/ml Oxacillin and incubated for 24 hours at 37[degrees] C to determine their sensitivity to the antibiotic.
Q Concerning the tube coagulase test
, I have always known to store (freeze) the bovine serum in glass tubes (12 mm x 75 mm).
aureus CoNS Coagulase Test
42(84%) 8(16%) MSA 45(90%) 5(10%) DNase 42(84%) 8(16%) Table 4.
These isolates were then re-identified by gram staining, Catalase test, Coagulase test
, DNAse test and preserved in 20% v/v glycerol in brain heart infusion (BHI) at-70AdegC till further use.
Speciation of CoNS was done based on Kloos and Schleifer classification and Koneman [1,2] by studying the colony morphology on nutrient agar, Gram reaction of smear, Haemolysis on blood agar, Mannitol salt agar, Catalase test, Coagulase test
, Modified oxidase test, nitrate reduction test, Voges-Proskauer test, Urease test, Oxidative fermentative test, DNAse test, Phosphatase test, Ornithine decarboxylase, Sugar fermentation-Glucose, lactose, Mannitol, Mannose, Trehalose, Xylose, Novobiocin and Polymyxin B 300 Susceptibility test.
Strain typing was done by biochemical methods including colony characteristics, Gram staining, and coagulase test
. Methicillin resistance was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines (M100-S23).6