Keywords: TP53 gene, Endometriosis, Codon
72, Pakistani population, Cell cycle.
Majority of isolates showed mutations in codon
531 are consistent with previous study from our country 19 However, contrary findings were shown in a study from Guangxi Zhuang, China showing highest mutations in codon
526 (50%) followed by 531 (35%) and in 516 (8%).
usage of the 13 protein-coding genes and the base composition of 37 genes for three species were analyzed using the program MEGA 4.
4%) with absence of WT8 band and presence of MUT3 band (indicating mutation at codon
S531L); 10 (1.
Polymerase chain reaction was carried out to obtain the desired DNA sequences (containing codon
12 and 13 of the K-RAS gene) using the designed primers, and the Big Dye Terminator, Applied Biosystem's buffer and water.
leprae susceptibility to rifampicin for first three cases (PAT 1, PAT 2 and PAT 3) which showed mutations at codons
424:GAA-GGA (Val-Gly) + 442:CAA CAC (Gln--His), 438: CAG--GAG (Gln-Val) + 442:CAA--CAC (Gln--His) and only at codon
442:CAA--CAC (Gln--His) respectively were performed in MFP assay.
Results: Significant associations between PCA risk and XRCC1 codon
399 polymorphism were found (such as A-allele vs.
Domestication of exogenous genes via contextualizing the codon
sequences for each specific host animal has been an accepted modification for overcoming cellular defense mechanism and subsequently the gene sequestration and silencing phenomenon.
MAS-PCR assay was performed by using two outer primers (Table 1) (11) to amplify a product of 435-bp (mutated allele) in the absence of the katG315 AGC codon
, and one inner reverse primer, binds at katG315 AGC second base and amplifies a product of 292-bp, (wild-type allele) in an authorized thermal cycler (Eppendorf Gradient Cycler).
As the table shows, the most common mutation in our patients was in codon
12, with substitution of Alanine instead of Glycine (GCT instead of GGT), which was present in 12% of the cases.
All known nucleotide mutations in codon
12 were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Translocation of the 30S ribosome one codon
farther along the tRNA is the function of elongation factor EF-G (EF-2 in eukaryotes) converting the ribosome to the post translation state and shifting the uncharged tRNA to the E site and the polypetidyl tRNA (the one carrying the incomplete polypeptide) to the P site.