cold buffer


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cold buffer

A reserved section of memory that has not received any data for a long period of time. Contrast with warm buffer. See buffer and buffer flush.
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After infection, cells were then washed once in PBS and lysed in cold buffer containing 50 mM Tris (pH 8.0), 110 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 50 mmol/L NaF and 0.1 mmol/L sodium vanadate.